1. Wavelength calibration
At the heart of spectrometer measurements is wavelength measurement, so wavelength calibration is crucial. The process of wavelength calibration can be carried out by calibrating the light source, usually using multiple light sources of different wavelengths such as tungsten lamp, mercury lamp, xenon lamp, etc. The specific steps are as follows:
Connect the fiber of the calibrated light source to the spectrometer, open the software and enter the wavelength calibration mode.
Follow the software prompts for wavelength calibration, and calibrate each channel in turn.
Once the calibration is complete, the relevant information is saved in the software and verified.
2. Sensitivity calibration
The sensitivity calibration is to ensure that the measurement sensitivity of each channel of the spectrometer is the same, so as to avoid measurement errors due to different sensitivities. The specific steps for sensitivity calibration are as follows:
Prepare the standard sample and measure on a spectrometer. The standard sample should have the same characteristics as the sample to be tested.
Compare the spectra of the standard sample with the experimental results, and adjust the gain or other parameters of the spectrometer to make the standard sample consistent with the experimental results.
Before sensitivity calibration, the channels in the spectrometer need to be processed, such as cleaned and adjusted.
3. Quality control calibration
Quality control calibration is to guarantee the accuracy of the measurement results during the use of the spectrometer. Here's how:
Prepare quality control samples and take measurements. Quality control samples should maintain constant characteristics, e.g., same concentration, stable composition, etc.
Compare the measured results of quality control samples with theoretical values, and adjust the measurement results by adjusting the parameters of the spectrometer, such as gain, integration time, etc.
Perform regular quality control calibrations and regularly replace vulnerable parts such as lamps and detectors in the spectrometer.