Environmental DNA sequencing technology can be applied to the monitoring of water, soil and atmospheric environment, which is an important means to assess the status of ecological environment and biodiversity, and is also an important tool in the field of environmental scientific research.
In order to jointly build an environmental DNA research ecology and focus on omics research hotspotsBased on MGI's DNBSEQ-G99 platform, Linn Biotech, a leading company in the field of environmental DNA testing in China, has launched the EDNA Metabarcoding (environmental DNA macro barcode) testing project.
EDNA (environmental DNA) refers to the DNA present in the environmental medium (soil, water, air), and in the environment in which we live, there may be DNA released by organisms everywhere (such as hair, mucus, saliva, urine and feces).
Figure 1 edna**。
The use of high-throughput sequencing technology to detect and analyze trace amounts of DNA released by each species in environmental samples can be used to detect the presence of species without damaging the ecosystem, or to identify the species of a specific species. Dynamic EDNA detection can also be used to study the dynamic changes of biodiversity and monitor the evolution of ecosystems. EDNA detection technology based on high-throughput sequencing has the characteristics of high sensitivity, high resolution, high efficiency and rapidity, and low sample volume requirements, and has become a powerful tool for studying the ecological environment, which will enable multiple application directions in the future.
Figure 2Application direction of EDNA detection technology.
Benefit from the advantages of the "speed king" DNBSEQ-G99 is fast, flexible, simple, and has a variety of read length optionsBased on the G99 platform, Linn Biotech has launched EDNA MetaBarcoding sequencing technology service, which improves the efficiency of EDNA detection, reduces the scientific research cost of related projects, and better serves China's environmental and ecological work on the premise of ensuring faster delivery of accurate and reliable detection data.
Product solutions
EDNA multi-sample type database building:Freshwater fish EDNA test in water samples, primer selection: Tele02, amplicon length 200bp.
Sediment samples were tested for EDNA of aquatic plants, primer selection: RBCl, amplicon length 520bp.
Sequencing protocol:MGI DNBSEQ-G99 (PE300 sequencing).
Analytical Scheme:LINN Biotech is equipped with EDNA analysis protocol.
Inspection process
Figure 3Schematic diagram of the detection process of EDNA metabarcoding sequencing technology.
EDNA was used to detect the detection of species
Whether it is a short amplification product (e.g., freshwater fish EDNA primer TELE02) or a long amplification product (e.g., aquatic plant EDNA primer RBCL), the G99 platform can detect the presence of species with high sensitivity.
Figure 4Taxonomic statistics at the EDNA species level (above: Aquatic Plant Research;Below: Freshwater fish study).
Note: The sequencing protocol and results** are from Linen Biotech.
In addition to shorter sequencing cycle and more efficient detection, the G99 platform can also meet a wide range of amplicon length detection types, especially for the detection and classification annotation of amplicons of longer fragments, which can meet the needs of multi-directional EDNA detection projects.
Based on the G99 sequencing platform, the EDNA project features
Nucleic acid extraction protocols for multiple sample typesThe EDNA metabarcoding detection project of Lingen Biotech has developed a characteristic EDNA nucleic acid extraction scheme for various types of samples such as water, soil, sediment, air, and animal digestive tract contents, which solves the technical bottleneck of extremely low EDNA content and difficult to obtain, and overcomes various types of EDNA research directions, which can provide efficient and stable EDNA research methods for the majority of ecological research scientists.
Rapid data delivery efficiencyThe EDNA Metebarcoding technology of Linen Biotech was carried out in conjunction with the "Speed King" G99 sequencing platformPE300 sequencing, the sequencing step can be completed in as little as 30 hoursIt can meet the research needs of EDNA amplicons of different lengths, is suitable for sequencing more types of EDNA primers, can quickly obtain sequencing data in a short time, greatly compresses the cost and time of scientific research, and provides a broader research space for scientific research users.
Upgrade EDNA analysis workflow and personalized database customizationLingen Biotech has been carefully polished for five yearsEDNA's proprietary analysis process。A larger and more comprehensive annotated database of fish species currently includes 18,686 freshwater fish species and 17,828 marine fish lists, and adds fish analysis of endangered species, detection and analysis of potential alien species, accompanying maps and descriptions in Chinese. More practical analysis content, more accurate detection, more suitable for Chinese scientific research users.
The output data output is stable, fast and accurateLinn Biotech introduced MGI's DNBSEQ-G99 sequencing platform, which adopts MGI's core DNBSEQ sequencing technologyAdvantages such as high accuracy, low repeats, and low label jumpsTo create an exclusive experimental and analysis process for the EDNA MetaBarcoding detection project of Lingen Biotech, to help scientific research users obtain accurate test results, accurately analyze the ecological composition, and monitor environmental changes in real time.
In addition to a variety of read length options such as PE300, the G99 sequencing platform perfectly solves the pain points such as difficulty in sample collection and long delivery cycle in sequencing services such as 16S with its extreme sequencing speed, flexible and simple on-board experience, and suitable throughput slide specifications. The wide range of application scenarios of G99 will continue to enable the rapid development of high-throughput sequencing technology in the field of ecological environment.