Ion exchange chromatographyIt is a chromatography technique that separates different biomolecules according to the difference in charge quality and quantity, and is divided into cation exchange and anion exchange resins according to the charged properties of functional groups. Since most of the biomolecules have acidic groups or basic groups, and their charged properties and quantities can be changed by adjusting the pH of the buffer, after the biomolecules are combined with the oppositely charged anion exchanger or cation exchanger, and the ionic strength or pH in the mobile phase is changed, the biomolecules can be eluted in order from weak to strong according to the binding capacity, so as to achieve the purpose of separation and purification.
In this paper, we take Borglon's ion chromatography resins as an example to introduce the application of ion chromatography resins and the characteristics of different types of resins.
1. Application of ion exchange resin
1.Applications of cation exchange resins:
Single antibodies, bispecific antibodies, and antibody-drug conjugates (ADCs).
Vaccines and virus-like particles.
Viral vectors, exosomes, lipid nanoparticles, etc.
2.Applications of Anion-Exchange Resins:
Single antibodies, bispecific antibodies, and antibody-drug conjugates (ADCs).
Enzymes, hormones, and blood products.
Vaccines, viral vectors, plasmids, and oligonucleotides.
Excellent DNA clearance in flow-through mode.
Features of ion exchange chromatography:
High resolution. High capacity. High yield.
Wide range of applications.
Good physical and chemical stability.
Simple cleaning-in-place.
Second, the selection of ion exchange resin
1.Ligand type
According to the charged nature of functional groups, they are divided into cation exchange and anion exchange, and according to their ionization in solution, they are divided into strong ion exchange groups and weak ion exchange groups, and the commonly used ion exchange groups are as follows
Strong ion exchange resins have the same ion exchange capacity and are fully charged over a wide range when the pH changes, while weak ion exchange resins provide different selectivity but are not suitable for extreme pH. When establishing a purification method, strong ion exchange resins are preferred after the ion exchange mode is determined, and weak ion exchange resins can be tried when the desired selectivity cannot be achieved with strong ion exchange resins and the expected resolution is not achieved.
2.Scaffolding type
The main difference between the base frames is the size of the packing pellet, which provides a difference in flow rate and resolution. Depending on the material of the scaffold, Borgron ion chromatography resins are available in the following categories:
Due to the different maximum flow rates tolerated by the resin, the resolution and load are different, and the purpose and requirements of each purification step are different, and the main purpose of the capture stage is to separate, enrich and stabilize the protein, and the load and flow rate are considered firstA large number of impurities need to be removed in the intermediate purification stage, and both capacity and resolution should be taken into accountThe removal of trace impurities or substances that closely resemble the sample of interest in the polishing stage requires the availability of the highest possible resolution resin. At the same time, sample volume and purity vary greatly during the purification process, and it is necessary to select the appropriate resin in combination with the specific purification step.
The specific parameter information of the relevant filler can be viewed on the official website of Borglon.
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