Ginseng fruit tissue culture room planning.
1. Site selection: The ginseng fruit tissue culture room should be selected in a location with flat terrain, quiet environment, convenient transportation and good infrastructure. At the same time, considering the special requirements of tissue culture experiments for environmental conditions, we should try to choose a site that is far away from pollution sources, has sufficient sunlight and has a good natural environment.
2. Spatial layout 1Experimental area: including operating table, microscope, culture rack and other facilities for experimental operation and plant culture.
2.Buffer room: The transition area before entering the experimental area, which is used to change the laboratory coat, shoes, etc., to avoid bringing external pollution into the experimental area.
3.Reagent storage area: store all kinds of reagents and medicines needed for experiments.
4.Media preparation area: The area used to prepare and sterilize the medium.
5.Washing area: The area used to clean the lab utensils and culture utensils.
6.Office area: The area used for the laboratory's office, meetings, and data archiving.
3. Equipment configuration 1Microscope: used to observe the structure and growth of plant cells.
2.Incubator: Used to place flasks to ensure that the medium in the flasks is evenly heated.
3.Incubator: Provide suitable temperature and humidity conditions to simulate the natural environment to promote plant growth.
4.Shaker: Used to shake the flask during the culture process to promote gas exchange.
5.Sterilizer: Sterilize the medium and utensils to prevent contamination by miscellaneous bacteria.
6.Ultra-clean workbench: provide a clean operating environment to prevent external pollution.
7.Refrigeration equipment: storage of perishable items such as culture media and reagents.
8.Waste gas treatment equipment: treat the harmful gases generated in the experimental process to protect the environment and personnel health.
9.Wastewater treatment equipment: treat the wastewater generated during the experiment to ensure that the discharge is up to standard.
Fourth, air purification 1Indoor air is filtered with a filter or air purifier to remove dust particles.
2.Control the direction of indoor airflow to avoid cross-contamination.
3.Strictly disinfect and clean the personnel and items entering and leaving the laboratory to prevent external contamination from being brought into the room.
5. Temperature and humidity control1Install temperature and humidity sensors to monitor indoor temperature and humidity in real time.
2.Equipped with an air-conditioning system to adjust the indoor temperature as needed to keep the temperature stable.
3.Control the indoor humidity with a humidifier or dehumidifier to meet the needs of plant growth.
6. Lighting and illumination1Choose the right artificial light source to provide stable lighting conditions.
2.Control the light intensity and timing to simulate the light conditions required for plant growth.
3.Lighting equipment such as shadowless lamps or reflectors is used to ensure adequate and uniform illumination of the operating area.
Start planning my 2024 4Equipped with curtains or blinds to adjust the light transmittance and avoid direct sunlight from interfering with the experiment.
7. Sterilization and disinfection1Items entering the laboratory are strictly disinfected to ensure sterility. It can be disinfected by ultraviolet lamp irradiation, alcohol wiping and other methods. 2.Regularly sterilize the laboratory table, floor and other areas to keep the environment clean and sterile. It can be sterilized by chemical disinfectants or ultraviolet sterilization.