Reagents |Overview of the basics (partial):
Chinese name: af532 maleimide.
Product Name: AF532 MAL, Alexa Fluor532 Maleimide
Fluorophore labeling: Alexa Fluor 532 dye.
Reactive group: maleimide.
Reactivity: thiol groups on proteins and ligands, oligonucleotide phosphorothioates.
Excitation of the conjugate Emission wavelength: 528 552 nm
Specification standard: 10mg, 25mg, 25mg, can provide mg-level and kg-level product development services.
Reagent manufacturer: Shaanxi Xinyan Bomei Biotechnology***
Reagents |Spectra:
af532 mal
Reagents |Reaction Mechanism (Partial):
AF532 MAL is a fluorescent dye that reacts with thiol groups in biomolecules to produce products with fluorescent properties. This fluorescent product can be detected by equipment such as fluorescence microscopy or flow cytometry to study the localization, distribution, and dynamics of biomolecules.
The advantage of AF532 MAL is that it has good sensitivity and specificity, and is able to react with thiol groups. In addition, AF532 MAL has good photostability.
AF532 MAL can be used to study protein interactions and localization, as well as protein transport and distribution within cells. In addition, it can be used to study biological processes such as apoptosis, cell cycle, and signaling.
Reagents |Attention to detail (partial):
1.Solid powder.
2.Soluble in most organic solvents, soluble in water.
3.Purity standard 95%+
4.Storage recommendations: Avoid repeated freeze-thaw cycles, store away from light, stock solutions should be used immediately, and any unused solution should be frozen in small aliquots at < 20°C
5.Caution: For scientific research purposes only.
Reagents |Related Reagents (Partial):
af488 aldehyde
af488 cho
af430 amine
alexa fluor430 nh2
af532 acid
alexafluor 532 cooh
af594 amine
alexafluor594 nh2
af514 nhs ester
alexa fluor514 se
af488 amine
alexa fluor488 nh2
af514 carboxylic acid
af514 cooh
af546 nhs ester
alexa fluor546 se
af555 nhs ester
alexa fluor555 se
af532 nhs ester
alexa fluor532 se
af750 nhs ester
alexa fluor750 se
This article is compiled and shared from: Dongdong of Shaanxi Xinyan Bomei Biotechnology***.