Reagents |Overview of the basics (partial):
Product Name: AF488 Labeled Streptavidin Conjugate, Alexa Fluor Labeled Streptavidin Conjugate.
Excitation wavelength (nm): 499
Emission wavelength (nm): 520
Specification standard: 10mg, 25mg, 25mg, can provide mg-level and kg-level product development services.
Reagent manufacturer: Shaanxi Xinyan Bomei Biotechnology***
Reagents |Packing:
Reagents |Reaction Mechanism (Partial):
AF488-labeled streptavidin conjugate combines the functions of the fluorescent dye AF488 and streptavidin. This conjugate can be used in experiments such as immunohistochemistry, immunofluorescence staining, and western blotting.
AF488 is a fluorescent dye with good photostability and fluorescence intensity. Streptavidin is a protein with good affinity and specificity that binds to biotin molecules. By conjugating streptavidin to AF488, a reagent with good affinity and fluorescent labeling can be prepared.
The preparation of AF488-labeled streptavidin conjugates typically involves the following steps: conjugating streptavidin to a biotin molecule to form a streptavidin-biotin complex;The fluorescent dye AF488 is combined with a biotin molecule to form the AF488-biotin complex;The streptavidin-biotin complex was conjugated to the AF488-biotin complex to form the AF488-labeled streptavidin conjugate.
This conjugate has a wide range of applications, such as in immunohistochemistry experiments, where it can be combined with antibodies to detect antigens in tissues or cellsIn immunofluorescence staining experiments, it can be combined with antibodies to detect antigens on the surface of cells or antigens inside;In western blotting, it can be conjugated to antibodies and used to detect protein-specific interactions.
Reagents |Attention to detail (partial):
1.Solid powder.
2.Soluble in most organic solvents, soluble in water.
3.Purity standard 95%+
4.Storage recommendations: Avoid repeated freeze-thaw cycles, store away from light, stock solutions should be used immediately, and any unused solution should be frozen in small aliquots at < 20°C
5.Caution: For scientific research purposes only.
Reagents |Related Reagents (Partial):
AF488 NHS ester is structurally identical to Alexa Fluor 488 NHS ester.
AF488 NHS ester is structurally identical to Alexa Fluor 488 NHS ester.
AF488-labeled sheep anti-mouse immunoglobulin (H+L).
AF488-sheep anti-rabbit immunoglobulin (H+L).
AF488 Tyramine.
AF488 NHS ester ultra-pure grade AF488 is structurally identical to Alexa Fluor 488.
AF488 NHS ester ultra-pure grade AF488 is structurally identical to Alexa Fluor 488.
AF488 tetraazine.
AF488 aldehyde is structurally identical to Alexa Fluor488 aldehyde.
AF488 aldehyde is structurally identical to Alexa Fluor488 aldehyde.
af488 tco
This article is compiled and shared from: Dongdong of Shaanxi Xinyan Bomei Biotechnology***.