The standardized pilot project of R D and Application of Light Sheet Illumination Microscope was a

Mondo Science Updated on 2024-01-28

Recently, the acceptance expert group of the enterprise standardization pilot project visited Linghai Life Science to carry out the acceptance of the Shanghai enterprise standardization project.

At the project acceptance report meeting, the expert group listened to the report on the work of the standardization pilot project, conducted on-site inspections on the research and development and application of the Gonghai light sheet microscope, and carefully checked the relevant information ledger of the standardization pilot project. The expert group highly affirmed the achievements and achievements of the standardization of life sciences in Gonghai, and unanimously agreed to pass the acceptance.

Introduction to the LS18 tiled light film display

The LS18 Light Sheet Microscope is a multi-functional tiled light sheet microscope with semi-automatic calibration capability, which can quickly tile multiple small and thin light sheets (up to 1 m thin) for segmented illumination at the same imaging field size. In addition, the LS18 light sheet microscope is suitable for multi-color rapid 3D imaging of sample tissues with transparent tissue by all tissue clearing methods, and the resolution can reach 70nm in combination with tissue expansion technology.

Features:

1. LS18 tiled light sheet microscope is adoptedUnique dynamic tiling technologyAchieving thinner sheet thicknesses with the same imaging field of view as other light-sheet microscopes, improving spatial resolution (x-y-axis resolution) and optical tomography capabilities (i.e., z-axis resolution), while enabling the microscope to automatically calibrate and optimize in real time.

2. The imaging mode is flexible and adjustable, and the LS18 light sheet microscope is equipped with different NA detection objectives to achieve a range from 063 times to 126x multi-level zoom observation is available in:Within minutesComplete imaging of the entire large sample tissue. Therefore, the LS18 light sheet microscope is suitable for high-speed 3D imaging at cell-level resolution in a wide range of large-scale tissues and organs.

15 min to quickly scan the intact mouse brain).

3. LS18 light sheet microscope withAll tissue clearing methods are compatible。It is suitable for imaging samples based on water-soluble, organic solvents, hydrogels and other clearing methods, as well as tissue imaging of different shapes and different mechanical strengths. 4. LS18 light sheet microscope has semi-automatic calibration function, reliable imaging quality, and easy maintenance. 5. LS18 light sheet microscope can perform multi-resolution, isotropic, multi-color three-dimensional imaging of transparent sample tissues

6. The LS18 light sheet microscope has a simple loading mode and is easy for users to operate.

Clamping method: Adhesive method.

7. The software interface of LS18 light sheet microscope is intuitive, and the operation steps are concise and clear.

8. There are a variety of sample fixation schemes to escort the complete imaging quality. According to the different shape, size, softness and hardness of the sample, the LS18 light sheet microscope has designed different sample fixation methods, such as adhesive method, clamping method, magnetic needle fixation, gel embedding and other schemes, and different types of transparent samples can be selected to make the sample fixed firmly and ensure the integrity and smoothness of sample imaging. At the same time, we have built a one-stop service platform to provide customers with professional one-stop scientific research services such as biological tissue clearing, immunostaining, 3D fluorescence imaging of tiled light sheet microscopy, data analysis, data storage, etc., aiming to provide personalized and customized solutions for every life science worker through accurate, fast and diversified scientific research services.

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