Compared with traditional animal models, cell culture models in vitro are relatively easy to operate, and the experimental results are relatively reproducible. Since the 50s of the 20th century, fetal bovine serum has been widely used in vitro cell culture worldwide as a commonly used and efficient additive.
Fetal bovine serum is a very complex mixture formed by the removal of fibrin from fetal bovine plasma, containing various plasma proteins, peptides, fats, carbohydrates, growth factors, hormones, inorganic substances, etc., most of its components are known, but some are still unclear, these substances to promote cell growth or inhibit growth activity is physiological balance. It is free of cells, fibrin and clotting factors. Fetal bovine serum is generally superior to other types of serum in terms of cell culture. Because fetal cattle have never been exposed to the outside world, antibody levels are significantly lower than in newborn and calf serum, so the risk of cross-reactivity between antibodies and cultured cells is also low, making it an ideal cell growth supplement.
A recent study has clarified the relationship between cell culture and fetal bovine serum.
In this study, there was no significant difference in the number of cell clonings, the number of cells and the cell viability of SP2 0 cultured with domestic or imported fetal bovine serum (P 0.).05) This is similar to the results of existing studies The reason may be that sp2 0 belongs to the same cell line as MDCK, some of the genetic material has been changed, and it has the characteristics of cancer, which can be passaged without restriction under in vitro culture conditions, and the dependence on serum is reduced, so it can grow and proliferate well in various types of serum The study also found that the number of cell clones, the number of cells and the viability of porcine fetal fibroblasts cultured by imported fetal bovine serum were significantly higher than those of domestic fetal bovine serum (P<001) Some researchers used different **fetal bovine serum to culture bone marrow mesenchymal stem cells, and found that in the process of using FBS1 culture, bone marrow mesenchymal stem cells gradually died, while FBS2 cultured bone marrow mesenchymal stem cells can not only quickly adhere to the wall and proliferate rapidly, but also maintain a strong ** proliferation ability after subculture It is speculated that it may be because pig fetal fibroblasts and bone marrow mesenchymal stem cells belong to primary cells and maintain biological characteristics in vivo due to different ** Fetal bovine serum, its collection methods and production and processing technology are different, and the composition and proportion of active substances such as cytokines are also different, resulting in the growth and proliferation of primary cells are greatly affected by serum**.
The quality of fetal bovine serum varies greatly among different **, even if it is the same brand and the same product number, there will be differences between batches due to the different gender, physiology, nutrition and other conditions of the blood supply animals. In this study, porcine fetal fibroblasts were used to test different batches of fetal bovine serum of the same brand and the same product number, and the results showed that the cell clones formed by FBS2-1 were small, and the number of cell clones was significantly lower than that of FBS2-2 and FBS2-3 (P<001）；After 10 days of culture, the number of cells and cell viability of FBS2-1 were significantly lower than those of FBS2-2 and FBS2-3 (P<001), although the number of cell clones in FBS2-2 was significantly lower than that of FBS2-3 (P<0.).01), but FBS2-2 has large cell clones and a significantly high number of cells.
FBS2-3(P<001) FBS2-2 was screened as a suitable serum for porcine fetal fibroblasts.
It can be seen from this experiment that different cells have different requirements for fetal bovine serum, and the culture effect of different batches and brands of fetal bovine serum is not the same.