First, the purpose of the experiment.
The purpose of this experiment was to provide a basis for further research on the relationship between plant melanism and plant growth and development by understanding the nature, function and detection methods of plant melatonia.
Second, the principle of experiment.
Melatonia is a hormone substance produced in plants, which has the effect of regulating plant growth and development. In this experiment, melatonization in plant tissues was extracted and quantitatively analyzed by spectrophotometer to determine the amount of melatonization.
3. Experimental procedures.
1.Plant tissue extraction.
Select a certain amount of plant tissue, grind it into powder with liquid nitrogen, add an appropriate amount of extract (such as methanol, ethanol, etc.), stir it well, and let it stand overnight. The next day, filter with filter paper and collect the filtrate.
2.Melato purification.
The filtrate is purified through a silica gel column to remove impurities. The purified solution is dried by a vacuum desiccator to obtain a pure melanistic product.
3.Standard curve preparation.
Different concentrations of melatonized standard solutions were prepared, and the absorbance was measured at a specific wavelength using a spectrophotometer to plot a standard curve.
4.Sample determination.
The purified melatonization sample was dissolved in an appropriate amount of solvent, the absorbance was determined according to the method prepared according to the standard curve, and the content of the melatonization was calculated according to the standard curve.
Fourth, the experimental results.
1.Standard curves.
By plotting a standard curve, determine the relationship between the amount of melancholic and the absorbance. The standard curve has a good linear range and can be used for quantitative analysis of samples.
2.Sample measurement results.
According to the standard curve, the amount of melanization in the sample was calculated, and the experimental results were obtained.
5. Experimental conclusions.
Through this experiment, we successfully established a method for the detection of plant melatonia, which provided an effective means for further research on the relationship between melancholica and plant growth and development. At the same time, the experimental results also showed that there were differences in the content of melatonia in different plant tissues, which may be related to the species of plants, growth environment and other factors. In the future, the effects of these factors on the melancholic content of plants can be further studied, so as to provide a theoretical basis for agricultural production.