AF700 NHS ester is a high-performance reagent used in a variety of applications, including labeling of biomolecules and detection of bioactive substances. AF700 NHS ester is a fluorescent dye that is frequently used in labeling reactions due to its high quantum yield and stability. It has excitation and emission maxima at approximately 680 nm and 700 nm, respectively, which allows detection using a variety of imaging techniques.
One of the most common applications of AF700 NHS ester is antibody labeling for flow cytometry. In this application, a standard conjugation reaction is used to conjugate a dye to an antibody to produce a labeled antibody that specifically binds to a target cell or antigen. The labeled antibodies can then be used to detect and analyze cell populations based on their antigen expression levels.
AF700 NHS ester can also be used in other labeling reactions, such as labeling of proteins, nucleic acids, and other biomolecules. The versatility of this dye allows it to be used in a variety of experimental settings, including biological, medical, and analytical chemistry studies.
In addition to labeling reactions, AF700 NHS esters can also be used to detect biologically active substances such as enzymes and small molecules. This is achieved by conjugating the dye to a substrate or ligand that binds specifically to the species of interest. The resulting conjugates can then be used to detect and analyze target species based on their binding affinity and specificity.
Purity: 95%+
Storage conditions: Protect from light, dry, -20 storage.
Precautions: Be sure to dry when taking and avoid frequent dissolution and freeze-drying.
Tips: It is only used for scientific research or industrial applications, and is not medicinal or edible.
The above is the relevant introduction of Shaanxi Xinyan Bomei on AF700 NHS ester.