Fast electrophoresis buffer is a buffer solution used in the electrophoresis process, which has the effect of maintaining a suitable pH and making the solution have a certain conductivity. It usually contains a high concentration of ions to facilitate the migration of DNA molecules.
There are many types of fast running buffers, such as TAE being the most widely used buffer system. The TAE buffer consists of trimethylolaminomethane (TriS) and ethylenediaminetetraacetic acid (EDTA), which is suitable for various DNA molecules, such as PCR products, cloning vectors, etc.
In addition, there is TBE buffer, which is suitable for agarose gel electrophoresis. The TBE buffer is composed of Tris, boric acid, and EDTA for high buffering capacity and resolution.
It is important to note that different buffers are suitable for different electrophoresis conditions and purposes, so the appropriate buffer should be selected on a case-by-case basis.
Electrophoresis time 25 minutes, voltage 250 V
Can be used in place of laemmli buffer.
High protein transfer rate.
Comparison of electrophoresis time versus temperature rise.
This product reduces running time by up to 60% compared to Laemmli buffer and increases the buffer temperature to almost the same extent as Laemmli buffer.
Comparison of protein transfection rates.
Since the Rapid Running Buffer Solution uses a lower gel concentration*1 than the Laemmli Running Buffer, the protein has a higher transfer rate.
1 Rapid Running Buffer Solution On low-concentration gels, high-concentration gels can be separated. For example, the separation of the Rapid Running Buffer Solution on an 8% gel is equivalent to that of Laemmli Buffer on a 12% gel.