With the development of modern medical technology, absorbable sutures are more and more widely used in surgical procedures. Compared to traditional metal sutures, absorbable sutures can be degraded within the body without the need for secondary surgical removal, reducing the patient's pain and recovery cycle. In this article, we will introduce the in vitro and in vitro degradation test methods and procedures of absorbable sutures, in order to provide a scientific basis for clinical application.
1. Preparation before the test.
1.Suture selection: Choose reliable and clinically proven absorbable sutures, such as polyester sutures, hydroxyapatite nanofiber filaments, etc.
2.Experimental sample preparation: In the in vitro test, select a sample of the same specification as the suture used in the actual surgery;In in vivo trials, animal models or in vitro substitute organs are used.
2. In vitro degradation test methods and steps.
1.Prepare buffers: According to the requirements of the test, select appropriate buffers (such as normal saline, PBS, etc.).
2.Place the suture sample in buffer: Depending on the experiment, the suture sample can be soaked in solution or fixed on a surrogate organ.
3.Set the test conditions: Set the appropriate temperature and time according to the test requirements.
4.Monitor sample degradation: Regularly monitor changes in the appearance and mechanical properties of suture samples, such as strength, elongation at break, etc.
3. In vivo degradation test methods and steps.
1.Animal model selection: Select the appropriate animal model according to the purpose of the experiment.
2.Surgical operation: According to the routine surgical procedure, the suture sample is implanted into the animal. Pay attention to the aseptic procedure during the operation.
3.Processing samples: At predetermined time points after surgery, a sample of sutures implanted in the body is removed.
4.Observation and documentation: Observe the degradation of the sample and compare the differences between the results of the in vitro test.
4. Data analysis and interpretation of results.
1.Organize experimental data: Organize and record the data obtained during the experiment.
2.Data analysis: The experimental data were statistically analyzed, and the degradation rate and intensity of sutures under different conditions were compared.
3.Interpretation of results: According to the experimental results, the in vitro and in vitro degradation performance of sutures was judged, and its safety and applicability were evaluated.
Conclusion: Through the use of in vitro and in vitro degradation test methods, the degradation performance and durability of absorbable sutures can be evaluated, and the scientific basis for their clinical application can be provided. In future research, we can also improve the biological function of absorbable sutures by adding a variety of bioactive substances, and further enhance its application value in surgery.