Exosomes are membranous vesicles about 30-150nm in diameter secreted by living cells that occur naturally in body fluids, including blood, saliva, urine, and breast milk. Tiny vesicles with lipid bilayer membranes carry and transmit important signaling molecules, forming a new intercellular information transmission system, which affects the physiological state of cells and is closely related to the occurrence and progression of a variety of diseases. Exosomes contain cytosolic proteins, proteins involved in intracellular signal transduction, various metabolic enzymes, heat shock proteins, tetratransmembrane proteins and special proteins, which are involved in the important regulation of cellular activities and are expected to be early diagnostic markers for a variety of diseases. Exosomes have become a hot topic in the study of gene and drug delivery, tumor tumors, and tumor biology. Exosomes are extracted by ultracentrifugation, and characterized and detected by electron microscopy, particle size and protein are the exosome identification methods recommended by mainstream journal articles.
Biothera exosome detection platform, based on the team's rich testing experience, provides ultra-high valence exosome analysis services from extraction to identification.
Service Content
1 Exosome Extraction Service.
2 Exosome electron microscopy testing services.
3 Exosome particle size testing service.
4. Exosome gene and protein detection and analysis services.
Exosome microRNA detection and analysis.
Exosome LNCRNA detection and analysis.
Detection and analysis of exosomal protein expression.
5 Exosome NSG sequencing services.
6 Delivery. Electron microscopy at different magnifications**.
Related experiment reports.
Sample requirements
l Serum, plasma requires 5ml sample, -80 cryopreservation, dry ice transport.
l Cell supernatant 20ml sample, -80 cryopreservation, dry ice transport.
l Saliva 15ml sample, -80 cryopreservation, dry ice transport.
l Urine 100ml sample, -80 cryopreserved, dry ice transport.
Fields of application
l Extracellular vesicles can promote the occurrence in vivo and control metastasis formation and immune response.
l In cardiovascular diseases, it can be used as a carrier for cardiovascular system transmission and has a pathological effect on vascularization.
l Exosomes in urine can be used as potential biomarkers of kidney disease, reflecting pathological changes from podocytes to renal tubules.
l In diseases and neurodegenerative diseases, it will also be used as a carrier of related disease proteins, and become a potential biomarker for early diagnosis.
servicesAdvantages:
Biothera team has mature testing experience and can provide a variety of service advantages:
l A variety of extraction schemes to meet different needs;
l Full range of characterization: NTA, electron microscope, WB, nanoflow cytometry one-stop solution.
l Strong professionalism: according to the specific analysis of customer experiments, to meet different experimental needs, to provide a variety of library construction services;
l Professional technical support, to ensure strong, thoughtful pre-sales and after-sales service support, ready to answer any questions for you.
Service process
After the two parties communicate with each other, determine the experimental plan, determine the service requirements, sign the contract, make an advance payment, start the experiment, and deliver the results.