As a biomaterial with unique fluorescence properties and good biocompatibility, FITC-dextran has a wide range of application prospects in the fields of biology, biomedicine and clinical applications. In the field of molecular biology and biotechnology, FITC-dextran is a very important biomaterial, which is dextran labeled by fluorescein (FITC). Due to its unique fluorescence properties and biocompatibility, FITC-dextran is widely used in many biological and biomedical studies.
The preparation of FITC-dextran generally consists of three steps: dextran acquisition, fluorescein labeling of dextran, and product purification. First, dextran can be obtained by microbial fermentation or chemical synthesis. Then, during the fluorescein labeling stage, dextran reacts with fluorescein molecules to form FITC-dextran. Finally, the product is separated and purified by chromatography purification technology.
FITC-dextran has a number of advantages. First, due to its fluorescence properties, it can be observed and tracked using a fluorescence microscope. Secondly, FITC-dextran has good biocompatibility and is non-toxic or low-toxic to cells and organisms, so it can be used for in vivo and in vitro experiments. In addition, FITC-dextran has good water solubility and high molecular weight, making it very useful in western blotting, DNA sequencing, and other molecular biology applications.
In biological research, FITC-dextran is often used in cell membrane permeability experiments. Due to its ability to cross the cell membrane and enter the cell, biomolecules such as intracellular proteins or nucleic acids can be labeled using FITC-dextran. In addition, FITC-dextran can also be used in the study of apoptosis, cellular immunity, and phagocytosis.
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