Recently, the top international journal of cardiovascular diseases, Circulation**, published a joint research article by Professor He Ben's team from Shanghai Chest Hospital and Zhou Bin's research group at the Center for Excellence in Molecular and Cell Science (Institute of Biochemistry and Cell Biology) of the Chinese Academy of Sciences diseases。 In this work, a dual-homologous recombination system was used to construct a technology that can capture the proliferation of endothelial cells in vivo for a long time, and to map the proliferation of aortic endothelial cells in homeostasis, hypertension and diabetes states, revealing the differences in endothelial cell proliferation between normal and disease states, and providing new ideas for the prevention of vascular complications of hypertension and diabetes.
The endothelial cells of the human body are like a thin veil of wool that covers the inner side of the entire cardiovascular system, similar to the lining of clothing. Although it is a thin layer, its integrity plays a very important role in the maintenance of the entire cardiovascular system. Endothelial cell injury is the initiation link of many diseases, including arteriosclerosis and myocardial infarction.
The integrity of endothelial cells is susceptible to hypertension, diabetes, hyperlipidemia, and inflammatory responses, and when endothelial cells are damaged and lost faster than they proliferate or renew, vascular endothelium is disrupted, and many cardiovascular diseases erupt. However, due to the long-term lack of reliable mapping methods, it is urgent to understand which parts of the endothelium are proliferating and where the endothelial damage is severe and need to be supplementedThere's no way to know. In response to this pain point, the research team used cell proliferation lineage tracer mice to label endothelial cells that underwent proliferation events in mice, making it possible to find proliferating endothelial cells, providing a solution for further clinical targeted intervention. It is of great significance for the prevention of hypertension, diabetes and coronary heart disease.
Li Yi, a postdoctoral fellow at Shanghai Chest Hospital, Liu Zixin, a doctoral student at the Center for Excellence in Molecular and Cell Science, Chinese Academy of Sciences, Han Ximeng, a postdoctoral fellow at Shanghai Chest Hospital, and Liang Feng, a doctoral student, are the co-first authors. Professor He Ben from Shanghai Chest Hospital and researcher Zhou Bin from the Center for Excellence in Molecular and Cell Science, Chinese Academy of Sciences are the co-corresponding authors. This research was supported by the National Natural Science Program of China and the National Key Research and Development Program of China.
Xinmin Evening News reporter Zuo Yan.