sulfo-cyanine3 dbco(Cyananine dye derived from Star Grey).It has many advantages as a reactive fluorescent dye in cell imaging applications, making it a tool in biomedical research. The following are the main advantages of sulfo-cyanine3 dBCOs in cell imaging applications:
1.Copper-free click reaction:Sulfo-Cyanine3 dbCo can be specifically conjugated to biomolecules containing azide functional groups by copper-free click reactions, which are highly specific and fast reaction rates that do not require organic solvents or complex experimental conditions, and are therefore suitable for labeling and imaging in live cells and live animals.
2.Water solubility:Sulfo-Cyanine3 DBCO has a sulfonic acid functional group, which makes it exhibit good solubility and stability in a water-soluble environment. This allows sulfo-cyanine3 dbco to be labeled and imaged in organisms without affecting physiological processes in organisms.
3.Cell permeability:The chemical structure and fluorescence properties of sulfo-cyanine3 dbco make it highly permeable to cells, which can easily enter the cell and localize to specific subcellular structures or biomolecules within the cell.
4.Low cytotoxicity:Sulfo-Cyanine3 DBCO exhibits low cytotoxicity in cells and does not have a significant effect on the normal physiological processes of cells, which is beneficial for long-term cell imaging studies.
5.Reversibility:The click reaction of sulfo-cyanine3 dbco is reversible and can be dissociated from the conjugation by the addition of excess azide functional groups, thereby enabling regulation of controlled release and labeling, further increasing its flexibility in cell imaging applications.
In summary, sulfo-cyanine3 dbco has the advantages of high specificity, water solubility, low cytotoxicity, and cell permeability as an active fluorescent dye in cell imaging applications. These advantages make sulfo-cyanine3 dbco a tool for studying the dynamic processes of specific molecules within cells, gaining insight into cellular function and mechanisms. At the same time, when using sulfo-cyanine3 dbco, it is necessary to optimize the experimental conditions according to the experimental needs to ensure the accuracy and reliability of the experimental results.
cy7-hylauronic acid
cy3sulfo-cy3 cooh
cy5 hydrazide
cy7 azide
icg-hyaluronate
icg-tetrazine
Stargraydye] The above data are from the literature, and Xinggray has not been independently verified, for reference only!(The above is limited to scientific research experiments and laboratory environments).