cy5-nhs ester(Cyananine dye derived from Star Grey).is a fluorescent probe that is commonly used in a variety of applications in molecular biology research. The following are the main applications of Cy5-NHS ester in molecular biology:
Nucleic acid markers:Cy5-NHS ester can be used to label nucleic acid molecules such as DNA, RNA, and oligonucleotides. This marker is commonly used in experiments such as in situ hybridization, Southern blot analysis, nucleic acid sequencing, and PCR amplification to visualize and detect specific sequences.
Protein markers:Cy5-NHS ester can react covalently with amino acid residues (typically lysine) in proteins to introduce fluorescent tags into proteins. This is useful for western blotting, protein quantitation, protein-protein interaction studies, and mass spectrometry.
Cell Tracing:Cy5-NHS ester can be used to label and track molecules within cells. It is co-labeled with biomolecules, such as antibodies, drugs, or molecular probes, to study their distribution and dynamic changes within the cell.
Immunohistochemistry:Cy5-NHS ester can be used to label antibodies for staining immune tissue sections. It allows visualization of the distribution of a specific protein or antigen in tissues for the study of histology, cell biology, and pathology.
Protein Affinity Purification:Cy5-NHS ester-tagged proteins can be used in affinity purification experiments to help isolate and enrich proteins of interest.
Drug Delivery Studies:Cy5-NHS ester can be used to label drugs or nanodrug carriers to study their distribution in vivo, tissue permeability, and drug release behavior.
Molecular Imaging:Cy5-NHS Ester fluorescent probes are useful in molecular imaging studies, especially in small animal models, to quantify and track the distribution of specific molecules or cells.
In conclusion, Cy5-NHS ester is a versatile fluorescent probe that can be used to label and track a wide range of biomolecules, thus providing a powerful tool for molecular biology research. It has a wide range of applications at the nucleic acid, protein, and cellular levels.
Stargraydye] The above data are from the literature, and Xinggray has not been independently verified, for reference only!(The above is limited to laboratory settings).