Table of Contents
Introduction 3 Installation Preparation 4
Install 5 uses 7
Maintain 12 articles **labconco official website:
Chapter 1 Introduction
The Centrivap Centrifugal Concentrator and Cold Trap are used for the rapid evaporation and concentration of solutions from biological and analytical samples under heated and vacuum conditions. Centrifugation eliminates bursting and foaming in a vacuum operation and concentrates the sample to the bottom of the vessel. This allows the sample to be concentrated into a few microliters. It contains a 200W heater that heats the sample chamber during the process to accelerate evaporation. A synchronous output of the vacuum pump power supply controls the automatic start of the vacuum pump when the rotor reaches operating speed. A safety switch prevents this concentrator from running when the lid is in the open position, and a door lock prevents the rotor from opening the lid while it is running.
The microprocessor that controls the heater and motor provides excellent modularity and reproducibility. With a preset timer, the end of the run may be automatically indicated. Nine different programs can be stored, making it easy to repeat precise test parameters.
Chapter 2 Installation Preparation
Before installing the centrivap, the installation site needs to be prepared and a level, stable surface must be selected. There are good exhaust conditions, power requirements, etc.
Power requirements. The Centrivap centrifugal concentrator requires a dedicated, well-grounded power socket with a current capacity of 8 A.
The cold trap requires a dedicated, well-grounded power outlet with a current capacity of 8 A.
Site & Exhaust Requirements.
If working with toxic and flammable solutions, the centrifugal concentration system is best placed in a fume hood, or it is strongly recommended that the exhaust of the vacuum pump be in a fume hood. Although the optional two-stage well effectively reduces solvent emissions, we still require the exhaust of the vacuum pump to be carried out in a fume hood.
Vacuum pump requirements:
When processing aqueous solution samples, the vacuum pump requires: the exhaust speed is 113L min, and the ultimate vacuum is 02*10-3 mbar。
For volatile solvents, diaphragm pumps can be used with venting speeds of 42 l min and ultimate vacuum levels of 200 mbar.
Vacuum tubes use 05' pipes.
Chapter 3 Installation.
Unboxing. Subassembly.
Installation. Open the sample chamber urgently.
The centrivap centrifugal concentrator cannot open the lid and enter the sample chamber when the power supply is interrupted. If you must open the lid when there is no power connection, insert a small screwdriver or similar tool into the small round hole in the upper left behind the control panel to unlock the door and open the lid with one hand and the other.
Note: Never attempt to open the door lock or lid while the centrivap centrifugal concentrator is running.
The positioning of components and the connection of pipes.
The Centrivap centrifugal concentrator, the cold trap and the vacuum pump are positioned relative to each other as shown in the diagram or in opposite directions. The cold trap's refrigeration system dissipates heat from the exhaust air from the rear of the instrument, with at least 3' of space around it to ensure good air circulation during the operation of the cold trap.
Once the components are positioned, the systems are connected together using the vacuum tubes provided. With 0The 5' vacuum tube is connected to the rear exhaust output of the centrifugal concentrator, and the other end is connected to a connector on the cold trap cover. Take another tube with another fitting on the cold trap cover. The other end is connected to the "out" connector on the secondary trap. Take another vacuum tube from "The Secondary Trap" to the input of the vacuum pump and tighten these connections with a clamp.
Exhaust mounting. If the centrivap centrifugal concentrator does not fit in the fume hood, connect the output of the vacuum pump using the tube provided by the user, secure it with a clamp and place the other end in the fume hood or in the special ventilation equipment in the laboratory.
Power installation. Connect the power cord of the centrivap centrifugal concentrator and the cold trap to the power socket of the laboratory, connect the power cord of the vacuum pump to the power output of the centrifugal concentrator, the power switch of the vacuum pump is in the "on" state, and the vacuum pump will be controlled by the centrifugal concentrator.
Rotor mounting. Place the rotor on the spindle of the centrifugal concentrator and gently rotate the rotor so that the plug on the spindle is sewn together with the card slot on the rotor. At this point, the upper end of the shaft is flush with the center of the rotor.
Chapter IV: Use
Plan. Before operating, it is necessary to have a thorough understanding of the procedures and equipment operation. The unique properties of the centrivap are the perfect balance of heating, vacuum and centrifugal force, which is not established, which can destroy or lose part of the sample. Therefore, if you are new to the Centrivap Centrifugal Concentrator or want to run a new application, the following is helpful for your pilot run.
Selection of sample tubes.
Under normal circumstances, the sample volume should not exceed half of the sample tube, and the size of the sample tube should be selected according to the rotor and sample volume, and the sample tube should not be loose in the rotor. Different rotor models provide boreholes to fit different sample tube sizes. Refer to Appendix D.
Loading. Good mechanical equilibrium ensures the balanced operation of the centrivap centrifugal concentrator, so that if it is not operated at full capacity, the weight of the sample on the sample rotor should be correctly distributed to ensure good symmetry when loading.
Heating settings are recommended.
The evaporation rate obtained from the centrivap centrifugal concentrator is affected by a number of factors. They include the properties of the solvent, the temperature and the pressure of the vacuum system.
In general, the evaporation process is accelerated by increasing the operating temperature as much as possible without damaging the sample.
Time setting recommendations.
The centrivap centrifugal concentrator has two built-in timers. The "run time" shuts down the entire centrifugal concentrator after a user-defined period of time, aborting the rotation, turning off the heater, vacuum pump, and releasing the vacuum. In order to prevent some heat-sensitive samples from receiving excessive heat after the solvent has evaporated, the heater can be turned off before the centrifugal concentrator is turned off, and a time can be set in the "heater time" so that when the time is up, the heater will be turned off first, and the rotation and vacuum will not stop until the "run time" is up.
Appendix C's table: "Centrivap specifications" indicates the approximate time required to evaporate different types of solvents, but the exact running time can only be determined by experiments. The Centrivap Centrifugal Concentrator has an audible prompt at the end of the preset time and automatically shuts down.
heat boost" heater operation.
Some models of the Centrivap centrifugal concentrator are equipped with a second "Heat Boost" heater, which is located in the upper part of the sample chamber wall and provides additional heat to the sample for faster evaporation. This "heat boost" heater is controlled by a microprocessor to maintain the temperature at the bottom of the sample chamber and prevent overheating of the sample.
Centrivap Control Panel.
The control panel of the Centrivap centrifugal concentrator is shown below and is detailed.
Display - The liquid crystal display shows the setting parameters and the actual measurement conditions.
program -- Displays the current program number.
temperature1 - Displays the temperature 1 of the setting.
temperature2 - Displays the temperature 2 set.
Time1 – Displays the elapsed time at the set temperature of 1.
Time2 - Displays the running time at set temperature 2.
Refrig (Refrigeration) – Shows if the refrigeration is running.
Program buttons -- Frequently used programs are launched with one click.
Run Stop Button – Used to start or abort a run.
Preheat Cool Button – Set the temperature of the sample chamber before loading, pre-cooling or pre-heating the sample chamber.
Increase Button -- When pressed, increases the value of the last selected setpoint parameter.
Decrease Button - When pressed, reduces the value of the last selected setpoint parameter.
Set Point Select Button -- Press this key to select the parameters that need to be changed. As a hint, display arrows on the set parameter points.
Operate the Centrivap Control Panel.
Pre-heating. Press the "preheat cool" button to pre-cool or heat the sample chamber, which is displayed on the display:
Press the increase or decrease button until the SP (set point) reaches the set value, the temperature of the actual sample chamber (Act) is displayed on the right, and when the heater heats up, the next line will light up at the actual temperature. Press the "preheat" key again, or press the "run" key, to abort the pre-heating of the sample chamber.
Select an existing program.
Operating parameters can be stored in memory, so that the running program can be reproduced for a total of 9 programs. To select a program: Press the "Select" key until the arrow indicates the program number, indicating that this set point may be run or modified. Press the increase or decrease key to select the program number, when the program number is changed, all set points display the last entered parameters of the program.
Store frequently used programs in programs or 3, so that pressing "PROG 1", "PROG 2" or "PROG 3" can start the program directly, and the rotor, heater, timer and vacuum pump can be started with just one key.
Change the temperature set point.
Press the "Select" key so that the arrow indicates temperature1 or temperature2 at the temperature set point, use the increase or decrease key to change the temperature set point, the temperature can be set from -4 to 99, if it is displayed as "hi", this setting temperature is 100. The last input is stored in memory.
Change the run time or heating, cooling time setting point.
Press the "Select" key so that the arrow indicates time1 or time2 at the run time setting point, use the increase or decrease key to change the run time, the time can be set from 1 minute to 999 minutes, if it is displayed as "on", it means that the centrifugal concentrator is running continuously. The last input is stored in memory.
If there is a running period, press the "stop" button, and the timer will remember the time of abort, and press "run" again to resume the timer. If you are running "PROG 1", "PROG 2", or "PROG 3", press the "PROG 1", "PROG 2", or "PROG 3" key to reset the timer and restart a new run.
When running programs other than "PROG 1", "PROG 2", or "PROG 3", you can reset the timer by pressing and holding the start button for 5 seconds.
Run the Centrivap centrifugal concentrator.
Note: Never attempt to open the lid while the rotor is turning. Do not damage the mechanical structure of the lid lock. Do not use if the lid is cracked or shows other signs of damage.
The centrivap centrifugal concentrator should never be tilted while running.
If the instrument is configured with LabconcoAfter the sample is dried, the no-load pump is running normally 2-More than an hour, let the vacuum pump discharge the volatile acids and organic solvents that may remain in the pump to avoid corrosion of the vacuum pump. If the damage is caused by corrosion, it is not covered by the warranty.
Turn on the cold trap power switch and when it reaches the operating temperature, the "ready" indicator lights up.
Turn on the Centrivap centrifugal concentrator and select a program or set the Temperature 1, Time 1 and Temperature 2, Time 2 set point parameters.
Click the Preheat Cool button to set the pre-cooling and pre-heating temperature, and pre-cool and pre-heat the sample chamber as needed.
If the centrivap centrifugal concentrator is equipped with a "Heat Boost" heater and additional heating is required, turn on the "Heat Boost" heater.
Place the sample in the sample tube, normally, no higher than half the height, and place the sample tube into the rotor.
Put the good rotor into the sample chamber. Then close the lid.
Press "Run", if the setpoint parameter is displayed, it will transform to show the actual parameter, and press "Run" again. The "S" (Stop) in the display is changed to "R" (Run) and is displayed alternately with the program number. If you want to run the program or 3, simply press "Prog 1", "Prog 2", or "Prog 3" to quickly start the Centrivap centrifugal concentrator. The rotor starts, and when the rotor reaches operating speed, the vacuum valve closes and the vacuum pump runs.
The parameters of the set point can be changed at any time during the run, first using the "select" key to select the parameters and then modifying them with the increase or decrease keys.
If the elapsed time is set, there will be an audible prompt when the set time is reached, and all functions will be terminated.
If the centrivap centrifugal concentrator does not stop on its own, press "stop" to stop.
When evaporation is complete, wait for the rotor to stop, open the lid and remove the sample.
Operational precautions.
When the solvent evaporates in a centrifugal concentrator and is collected in a cold trap, the temperature of the cold trap rises due to the increased load.
Depending on the type of solvent used, sample volume, and system operating parameters, the cold trap temperature may rise to the point where the "ready" lamp is turned off, but when the sample enters the completion phase, the load on the cold trap drops, and the temperature of the cold trap drops, and "ready" lights up again.
The LCD display of the centrivap centrifugal concentrator conveys a wide range of information. When it turns on, it reverts to the same state it was in before the shutdown ("run" or "stop"). If it is in the "run" state when it is shut down, when it is turned on again, it will try to revert to the program setpoint before the shutdown and continue to run.
Outages. During operation, press the "STOP" key to interrupt the operation and turn off all operating functions. Once the rotor has stopped, the lid can be opened. If you want to rerun, close the lid and press "Run" and it resumes operation at the same set point, and the timer resumes timing from the time it was interrupted.
Chapter V: Maintenance
Under normal operation, only a small amount of maintenance is required. The following maintenance logs are recommended.
Daily:
Remove all spills: Remove the liquid from the sample chamber and clean all surfaces in a manner appropriate for spills.
Use a soft cloth, sponge or suede and moisten the lid and seal with mild soap or detergent.
Check the oil level of the vacuum pump, which should be between "min" and "max". If the oil level is lower than "min" on 1'(25.).4 mm), refueling to the appropriate position.
Diaphragm pumps do not need to check this item.
If the oil appears cloudy, particulate, or discolored, drain the original oil and replace it with fresh oil.
After running the acidic solution, clean and neutralize it immediately after the run, otherwise it will cause physical damage to the collector.
If a glass cold trap is configured, this can be omitted.
Check the solution collected or frozen in a cold trap and dispose of it appropriately. Empty the cold trap of the solution before the next run. If the solution is frozen in a glass trap, run on cold water immediately after operation.
If using a glass trap, check that the ethanol in the stainless steel trap is free of ice or water, release the ethanol, and replace it with fresh ethanol.
Monthly:
Inspect all rubber hoses and seals and replace damaged parts. The rubber components on the centrivap centrifugal concentrator may be permanently damaged and need to be replaced. The service life of these rubber components is related to their use rate and the surrounding environment.
Use a soft cloth, sponge or suede and moisten the lid with mild soap or detergent.
Use a soft cloth, sponge, or suede with mild soap or detergent to clean the exterior surface of the instrument. Liquid spray cleaners and polishes can be used on exterior surfaces, but do not use solvents to remove dirt from exterior surfaces, which may cause damage.
Annual:
1. Every year, clean the heat sink of the cold trap refrigeration system. If the operating environment is very frequent, it can be more frequent.