The Rabbit Resistin (RETN) ELISA Kit is a kit for the detection of resistin (RETN) content in rabbit serum, plasma, and related liquid samples. By using rabbit resistin (RETN) ELISA kits, researchers can quickly and easily quantify a large number of samples, providing strong support for further research on the role of resistin in animal physiological and pathological processes.
1. Kit composition.
The rabbit resistin,RET ELISA kit mainly includes the following components::
1.Microplate plates: used for sample loading and incubation, typically 96-well plates.
2.Enzyme-labeled antibodies: antibodies that specifically bind to rabbit resistin to capture resistin in a sample.
3.Enzyme-labeled secondary antibody: A secondary antibody that specifically binds to an enzyme-labeled antibody and is used to form an immune complex with the enzyme-labeled antibody.
4.Substrate: A chemical substance used in a chromogenic reaction.
5.Standards: Standards of different concentrations that are used to establish a standard curve.
6.Manual: Contains detailed operating procedures and precautions.
2. Steps.
The procedure for detection using the rabbit resistin (RETN) ELISA kit is as follows:
1.Add the standard and sample to be tested to the plate wells and incubate thoroughly.
2.Wash the plate to remove unbound components.
3.An enzyme-labeled antibody was added to bind to rabbit resistin in the sample.
4.After washing the plate, enzyme-labeled secondary antibodies were added to form immune complexes with enzyme-labeled antibodies.
5.The substrate is added and the chromogenic reaction is carried out.
6.The optical density value of each well is detected at a certain wavelength and the data is recorded.
7.The concentration of rabbit resistin in the sample to be tested was calculated according to the standard curve.
3. Precautions.
The following points should be noted when using the rabbit resistin (RETN) ELISA kit:
1.Make sure the kit is within the expiration date and in good condition, and avoid repeated freeze-thaw or prolonged exposure to high temperatures.
2.Read the manual carefully before use and operate according to the requirements of the manual.
3.Make sure the experimental area is clean and tidy to avoid cross-contamination.
4.Regularly check the performance of the instrument and equipment to ensure the accuracy of the test results.
5.If you encounter any questions or concerns during the experiment, please consult a professional or contact the kit provider in time.