Thierry Jaffredo, the Center for Cell Engineering and the French EFS Center for Cell Engineering, and Laurence Guyunneau-Harmand of the Sorbonne University have collaborated to generate transgenic-free hematopoietic stem cells (HSCs) using induced human pluripotent stem cells. The research results were recently published in the international academic journal Cell-Stem Cell.
Using an original one-step culture system, the researchers obtained carrier-free, matrix-free transplantable hematopoietic stem cells by differentiating human induced pluripotent stem cells (HIPSCs). Upon injection into immunocompromised mice, HIPSCs-derived cells colonize the bone marrow and form a robust multilineage hematopoietic population that can be transplanted serially. Single-cell RNA sequencing results showed that this replantation activity was generated by a hematopoietic population that was transcriptionally similar to that of human embryonic aortic ** hematopoietic stem cells.
The results of this study suggest that HIPSCs can generate hematopoietic stem cells and will help uncover the key regulators of hematopoietic stem cell production during human development.
According to the researchers, HSCs are rare cells that produce lifelong efficacy in hematopoietic cell (HC) transplantation. In recent decades, the demand for clinical-grade hematopoietic stem cells has increased dramatically, leading to difficulties for patients. The use of pluripotent stem cells to generate hematopoietic stem cells is a promising but unmet goal.