Derivatization of samples is an important pretreatment step in many scientific fields, including chemistry, biology, medicine, and environmental science. The main purpose of sample derivatization is to improve certain properties of the sample and make it easier to detect, analyze, and measure. Here are some reasons to pre-derivatize your samples:
1.Increased detection sensitivity: Some compounds may be difficult to detect directly because they are too low in concentration or similar in nature to the analyte of interest. By derivatizing the sample, the concentration of the compound of interest can be increased or differentiated from other compounds, thereby increasing the sensitivity of the assay.
2.Improved assay specificity: Sometimes, multiple compounds may be present in a sample that have similar properties and structures. By derivatizing the sample, the properties of the target compound can be altered to give it a unique characteristic, thereby improving the specificity of the analysis.
3.Simplified sample handling: Some samples may contain significant amounts of impurities and interfering substances that may interfere with the detection and analysis of target compounds. By derivatizing the sample, the effects of these interfering substances can be removed or reduced, simplifying sample handling.
4.Improved analytical methods: Some analytical methods may be limited and cannot be directly applied to the detection and analysis of certain samples. By derivatizing the sample, the properties of the target compound can be changed to fit existing analytical methods, thereby improving the applicability of analytical methods.
In conclusion, sample derivatization plays an important role in scientific research. It can help us better understand the nature and composition of samples, improving the accuracy and reliability of detection and analysis.