Construction of a PCR laboratoryAre you only focusing on the planning of the experimental area and ignoring the setting of the auxiliary area? Is the ventilation system simply installed without taking into account the diffusion of aerosols? In the pursuit of experimental efficiency, have you overlooked the importance of anti-contamination measures? Today, let's decipher the 4 points that are most easily ignored in the construction of PCR laboratories, so as to escort the construction of your laboratory and successfully accept it.
1. Unreasonable regional planning
The planning of the four areas of reagent preparation, sample processing, amplification and product analysis is very important to prevent cross-contamination and must not be abandoned because of neglect or lack of budget.
Second, the design of the ventilation system is unreasonable
The generation and diffusion of aerosols is a very important issue during PCR experiments. If the ventilation system is poorly designed, it can lead to aerosol diffusion, increasing the risk of aerosol contamination. Therefore, the design of the ventilation system should be personalized according to the specific layout of the PCR laboratory.
Third, pollution prevention measures are not in place
Cross-contamination of samples is a common problem, and it is easy to pay attention to the areas that are easy to overlook. It must not be caused by non-standard operation or insufficient training in the later stage.
Fourth, the safety equipment is insufficient
PCR laboratories need to be equipped with protective equipment such as biological safety cabinets and protective clothing to ensure safety. At the same time, these protective equipment should also be regularly inspected, maintained and replaced in the experimental work.
The most easily ignored points in the construction of PCR laboratory involve four aspects: unreasonable regional planning, unreasonable ventilation system design, inadequate anti-pollution measures, and insufficient safety equipment.