Plague antigen ELISA assay kit
Instruction manual
The plague antigen ELISA test kit is an experimental tool specifically designed for the detection of plague antigens. By using this kit, researchers can perform quantitative or qualitative testing of plague antigens in the laboratory. The kit typically consists of a microplate label, enzyme conjugate, wash solution, substrate, and standard.
The microplate is the core component of the kit and is typically made of polystyrene. It has many micropores that can be used to immobilize antigens or antibodies. Enzyme conjugates are enzyme markers that bind to antigens or antibodies, and by reacting with antigens or antibodies, they form detectable enzyme labeling complexes. The wash solution is used to remove unbound material and ensure the accuracy of the experimental results. The substrate is a chromogenic agent that reacts with an enzyme labeling complex to produce a color change that enables antigen detection. A standard is a known concentration of an antigen or antibody that is used to calibrate the results of an experiment.
When using the Plague Antigen ELISA Assay Kit, researchers need to follow certain procedures. First, the antigen or antibody is fixed on the plate and then the enzyme conjugate and wash solution are added. Next, a substrate is added and the color change is observed, and finally the antigen concentration is determined by colorimetry. Throughout the experiment, researchers need to tightly control the experimental conditions to ensure the accuracy and reliability of the experimental results.
Plague is an infectious disease caused by Yersinia pestis, which is highly contagious and lethal. Therefore, timely and accurate testing is essential to control the spread of the outbreak. As a reliable detection tool, plague antigen ELISA detection kit plays an important role in epidemic prevention and control. With the continuous development of technology, it is believed that this kind of kit will be more perfect and escort human health.