Mouse free thyroxine ELISA detection kit
Instruction manual
The Mouse Free Thyroxine ELISA Assay Kit is a kit specifically designed to detect the concentration of free thyroxine in the serum or plasma of mice. It uses enzyme-linked immunosorbent assay (ELISA) technology to sensitively and accurately quantify the amount of thyroxine in mice. The main components of the mouse free thyroxine ELISA detection kit include microplate plates, antibodies, standards, washing solutions, substrates, etc. The plate is used to support the binding reaction of the antibody to the sample, the antibody is the specific recognition molecule for thyroxine, the standard is used to establish the standard curve, the wash solution is used to clean the plate, and the substrate is the final color material. When using the mouse free thyroxine ELISA test kit, it is first necessary to collect a serum or plasma sample from the mouse, and then follow the requirements of the kit instructions for sampling, incubation, washing, and color development. Finally, the absorbance value of the sample was measured by microplate reader, and the concentration of thyroxine was calculated according to the standard curve. The mouse free thyroxine ELISA detection kit has the characteristics of high sensitivity, high specificity and high repeatability, and can be used for the research of mouse thyroxine-related diseases, such as hyperthyroidism and hypothyroidism. In addition, the kit can also be used to monitor the effect of drugs on thyroxine during drug development. In conclusion, the mouse free thyroxine ELISA detection kit is a powerful and easy-to-use experimental tool, which is of great significance for the research and drug development of mouse thyroxine-related diseases.