1. Separation method
1.1 The fermentation products of the six fungi were placed in centrifuge tubes with a high-speed centrifuge, centrifuged at 20 8000r min for 10 min, the supernatant was separated, and the collected mycelium was placed in a petri dish.
1.2. Vacuum pressure suction filtration method: The fermentation products of 6 fungi were respectively filtered at room temperature and decompression by suction and filtration device to separate the mycelium from the fermentation broth. The mycelium remains on the filter paper and the fermentation broth flows into the collection flask.
1.3 multi-layer gauze filtration method, the gauze after folding 8 layers is placed in a petri dish, the fermentation products of 6 kinds of fungi are spread on the gauze respectively, the operator wears sterile latex gloves, rolls up the gauze carefully, and tightens the gauze in reverse with both hands to separate the fermentation broth from the mycelium, and gently unfolds the gauze to collect the mycelium when there is no liquid flowing down.
2. Determination method
2.1 Conidia counting: Count the spores of the fungus under a microscope with a hemocytometer.
2.2. After the mycelium moisture content strain was cultured for a certain period of time, 100ml of fermentation products of different strains were taken respectively, and the mycelium was collected by three methods: high-speed centrifugation, vacuum filtration and gauze filtration, and the wet weight of the bacteria was accurately weighed ( ) and then the bacteria were dried to constant weight under 105 and the dry weight was accurately weighed.
2.3 After the mycelium and fermentation broth separated by high-speed centrifugation method were centrifuged by high-speed centrifugation, the six fungi were separated from the mycelium and fermentation broth, forming flocculent or loose precipitate. After separating the supernatant with a pipette, the mycelium was found in a Petri dish and was found to still contain a small amount of fermentation broth in the mycelium collected after centrifugation due to the fact that the mycelium was very light (only the mycelial bulbs of Globococcus were hard) and the fermentation broth was viscous.