The performance of the instruments and equipment in the laboratory is an important factor for the benign operation of the laboratory, but whether the instruments and equipment operate normally and play a role is closely related to whether the laboratory can use the instruments and equipment correctly, and whether they can be maintained and maintained in time. The daily maintenance and maintenance of instruments and equipment is an important part of equipment technical management, the purpose of which is to extend the service life of instruments and equipment, maintain their good performance and accuracy, and is a preventive and protective measure to ensure the normal operation of instruments and equipment to the greatest extent, and is the basis for ensuring the normal and smooth daily work of the central laboratory. Here, some of the instruments and equipment commonly used in the central laboratory and their daily use and maintenance will be listed.
1. Centrifuge.
An instrument that uses the centrifugal force generated by the rotating rotor to separate substances of different densities and particle sizes in a suspension or emulsion, or to analyze them at the same time as separation. A wide range of applications.
There are many types of laboratory centrifuges: according to the structure, it can be divided into benchtop centrifuges and vertical centrifuges (floor centrifuges); According to the separation method, it can be divided into filter centrifuge and sedimentation centrifuge; According to the speed, it can be divided into low-speed centrifuge, high-speed centrifuge and ultra-high-speed centrifuge; According to the capacity, it can be divided into microcentrifuge (microcentrifuge or mini centrifuge), small-capacity centrifuge, large-capacity centrifuge and ultra-large capacity centrifuge; According to whether there is refrigeration or not, it can be divided into refrigerated centrifuge and room temperature centrifuge.
a) Operating procedures of the centrifuge.
1) The centrifuge should be placed on a level and solid floor or platform, and strive to keep the instrument in a horizontal position to avoid oscillation of the instrument during centrifugation.
2) Turn on the power switch, place the pre-balanced sample symmetrically on the sample holder of the rotor, and close the cover.
3) Rotate the timing knob to set the centrifugation time, and slowly rotate the speed adjustment knob to make the speed of the instrument meet the predetermined requirements.
4) After centrifugation, adjust the speed adjustment knob back to the zero position and turn off the power switch.
5) When the centrifuge stops rotating completely, open the cover, take out the centrifugal sample, and close the lid again to end the centrifugation.
6) Cleaning of the centrifuge chamber: In order to avoid contamination of samples and other residues, the centrifuge shell and centrifuge chamber should be cleaned frequently. For the cleaning of the centrifuge chamber, the centrifuge cover should be opened first, the power cord should be unplugged, the centrifuge rotor should be unscrewed with special equipment, and then the centrifuge chamber should be cleaned with a neutral detergent (70% isopropanol or ethanol); The rubber seals in the centrifugation chamber are treated with detergent, rinsed with water, and lubricated with glycerin.
7) Rotor cleaning: The rotor will be contaminated by sample residues and may also be corroded by some chemical agents, so the rotor should be cleaned and maintained every month. Clean the rotor once a month with a mild detergent and keep a record in the instrument maintenance log book to extend the life of the rotor.
b) Centrifuge maintenance.
1) In order to ensure the safety of the experiment and good centrifugation, the instrument must be placed on a sturdy, level table, no items should be placed on the plastic cover door, the sample must be placed symmetrically, and the nut must be tightened before turning on.
2) The rotor and the centrifuge tube used for the test should be checked frequently for cracks, aging and other phenomena, and the centrifuge tube must be replaced in time if so.
3) After the test is completed, the instrument needs to be wiped clean to prevent corrosion.
4) When the length of the carbon brush of the motor is less than 6mm, it must be replaced in time.
5) Do not open the lid when the centrifuge is not stopped.
6) The instrument must have reliable grounding.
7) After the experiment, turn off the power switch at the back, and turn on the power switch at the back when you unplug the power plug.
8) Pay attention to the cleanliness of the instrument in daily use.
9) Shut down in strict accordance with the shutdown procedure of the instrument.