Cell Biology, Proteomics, and Clinical Pharmacy

Mondo Education Updated on 2024-02-17

4. Cell biology technology platform.

a) Electroporator.

Applications: It can be used to introduce biological macromolecules such as nucleotides, DNA, RNA, proteins, sugars, dyes and virus particles into prokaryotic and eukaryotic cells to achieve the purpose of gene transfer or introduction of foreign molecules. Compared to other physical, chemical, and viral methods, electroporation is a more effective method. Electroporators are widely used in molecular biology and cell biology research.

b) Tabletop gene gun.

Applications: Transfection of a wide range of biological samples, such as bacteria, fungi, insects, plants, and various animal cells.

c) Real-time label-free cell function analyzer.

Applications: It can detect dynamic biological reactions such as cell growth, proliferation, toxicity, adhesion and morphological changes in real time, and can support applications such as cell proliferation, cytotoxicity, cell adhesion and receptor signal detection.

d) Cell counter.

Application: For cell counting.

f) Fluorescence microscopy.

Applications: It is used to study the absorption and transport of intracellular substances, the distribution and localization of chemical substances, etc.

5. Proteomics technology platform.

a) Ultracentrifuge.

Application: Its maximum speed can reach 100000R min, and it can be used with different rotors for the separation and purification of biomolecules, and can be applied to the separation of virus and subcellular components, protein gradient separation, lipoprotein separation, RNA gradient precipitation, and plasmid DNA purification.

b) Chromatography system.

Applications: For fully automated purification of proteins and peptides.

3) Two-dimensional electrophoresis system.

Scope of application: Separate proteins according to different isoelectric points, and then separate them according to different molecular weights, so that different proteins can be separated as much as possible, and can be combined with mass spectrometry to study the proteome of cells, and what are the differences in protein expression.

6. Clinical pharmacy research platform.

a) High performance liquid chromatograph.

Applications: Qualitative and quantitative analysis of chemicals and natural products.

2) High-efficiency rotary evaporator.

Applications: For the preparation, extraction and separation of chemicals and natural products, as well as solvents**.

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