Animal experiments are an important means for basic and translational research of diseases, and the selection of appropriate animal models is also one of the important factors for the success of experimental research. Jiang Tao, a senior engineer at the Biology Research Center of the Institute of Genetics and Development, Chinese Academy of Sciences, said in an interview with a reporter from China Science News that the factors to be considered for experimental animals are: easy to raise, high reproductive rate, high genetics and degree of purity, metabolic type, physiology and pathology as close as possible to humans. Mice have a clear advantage on these points.
01. In the field of scientific research: mouse modeling to study the pathogenesis of diseases (including inflammation, tumor, infection, endocrine, digestive system, etc.) and the evaluation of the mechanism of drug activity.
02. In the field of transformation: mouse modeling has completed the preclinical research evaluation of drugs, including pharmacology, efficacy, toxicology, and safety evaluation.
Common disease models in mice.
In addition to observing the morphology, mouse experiments often need to collect plasma, urine, and cerebrospinal fluid to detect changes in physiological and biochemical indicators. However, due to the requirement of the amount of blood collected by animals each time, scientific experiments are limited to a certain extent.
Requirements for blood collection in laboratory animals, from: guidelines for blood collection in laboratory animals
The sample size of the experiment is not enough, which is precious.
Multivariate Screening Assays?
Cost savings and efficiency?
*Luminex multiplex assays, which require only 25 L of sample, can be quantified for up to 50 indicators.
Literature case sharing
01. Mouse models evaluate the safety of drug materials
A research team from Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, has previously developed a new calcium-phosphorus (CAP) usncs that is able to fully enter the tortuous subchannels of collagen fibers (about 18-4 nm), thus better restoring the function of osteoporotic bones, more advanced than the widely used hydroxyapatite nanoparticles (HANPS). To address the biosafety and potential clinical applications of USNCS, the research team experimented with mice and developed a model in BioActive Materials (if = 16874) A study** was published in the journal to quantify the expression of cytokines in serum using the R&D's Luminex Mouse Multifactor Assay Kit to assist in the assessment of the biosafety of novel nanoparticles (calcium-phosphorus (CAP) USNCs).
Product: LXSAMSM Luminex Mouse Discovery Assay Number of indicators: 18
The biosafety differences between CAP USNCS and HANPS in mice were investigated in contrast, and based on the characteristics of the immune response, histological assessment of major organs and local tissues, and a whole-body transcriptomics review, it was found that CAP USNCS was no more dangerous than FDA-approved HANPS. The results of this study are of great value for future clinical applications and introduce an innovative strategy for systemic response assessment.
02. Mouse infection model to evaluate immune inflammation
Human respiratory syncytial virus (HRSV) infection brings many adverse clinical outcomes to patients, ranging from mild colds to severe bronchiolitis and even acute interstitial pneumonia. Genetic background is one of the most frequently cited factors influencing clinical diversity. At the same time, some studies have found that the early experience of infection affects the severity of heterologous infection. To test this idea, Gautier Gillliaux et al. constructed different mouse infection models (mouse pneumonia virus (PVM), murine herpesvirus (MUHV-4)) to analyze the importance of two host-related factors (gene composition, immune activation) in shaping the host's immune response to pneumonia virus disease. R&D Systems Luminex Mouse multiplex assays were used to assess the expression of pro-inflammatory and chemokines in tissue homogenates of mice under different states of infection.
Product: LXSAMSM Luminex Mouse Discovery Assay Number of indicators: 22
The results of the experiment found that in the case of continuous vaccination with MUHV-4 and PVM, prior herpesvirus infection improved the immune system's response to subsequent pneumovirus primary infection in a genetic background-dependent manner. In addition, MUHV-4 provides significant innate immune priming (including redirecting and modifying alveolar macrophage cytokines and chemokines) in response immediately after exposure to PVM, thereby greatly attenuating the host's inflammatory response and disease severity.
03. Mouse eye modeling experiment
Retinal macular fibrosis is common in neovascular age-related macular degeneration (NAMD), and the main** method of NAMD is intraocular injection of VEGF inhibitors, but approximately 40%-70%** of patients develop macular fibrosis within 10 years, causing permanent macular structural destruction and irreversible visual damage. Aging is one of the most important factors leading to organ fibrosis, but the mechanism of increasing organ fibrosis through aging is not clear.
In February 2023, the team of Professor Xu Heping from Aier School of Ophthalmology and Aier Institute of Optometry, Central South University, published an article in the Journal of Neuroinflammation, **The effect of aging on subretinal fibrosis secondary to choroidal neovascularization and its mechanism. The R&D Systems Mouse Luminex multivariate assay was used to compare the cytokine secretion of macrophages (BMDAS) in bone marrow** in young and elderly mice with TGF-1** and young and elderly mice without subretinal fibrosis, and the results showed that BMDAS in older mice** developed towards a pro-angiogenic and pro-fibrotic phenotype, especially under disease conditions.
Product: LXSAMSM Luminex Mouse Discovery Assay Number of indicators: 15
The results showed that there was a retina-bone marrow-blood-retinal signaling pathway that recruited fibroblasts and macrophages during retinal injury repair, and the pro-fibrotic function of macrophages was increased with aging, and more circulating fibroblasts were recruited to increase omental macular fibrosis. This study elucidated the mechanism of omental fibrosis in aging and emphasized, and provided new ideas for the clinical development of secondary NAMD macular fibrosis.
Bio-Techne's new Mouse High Performance Series Luminex Multiplex Assay Kits offer improved performance and larger panels for high-quality screening and validation experiments. Greatly improve the detection efficiency in mouse precious samples and multi-index detection experiments!
Product name: Mouse XL Cytokine Luminex Performance 45-plex Fixed Panel
Catalog number: LKTM015
Product Advantages:
45 factors can be detected simultaneously in a single well, requiring only 25 l wells;
It is highly flexible and can be freely combined, and it is also the largest panel that can be freely combined with mice on the market.
The Mouse High Performance series has been correlated with the gold standard ELISA to ensure accuracy;
The indicators are comprehensive, including cytokines, chemokines, tumor endocrine, autoimmunity and other biomarkers;
High batch-to-batch consistency to ensure the stability of test results;
Research Field: Immunity, Inflammation, Infection, Vaccine, Drug Efficacy and Safety Evaluation, etc.
Metric breakdown
Cross-reactivity
The antigen was added to the assay solution at 3 times the standard concentration and tested for cross-reactivity. Cross-reactivity was < 0 for all antigens and 45 analytes in the panel5%。
Inter-batch Intra-batch CV value
Batch-to-batch accuracy: Average CV values calculated for 40 parallel experiments with 2 analytes at different concentrations in a single experiment.
Intra-assay precision: Average CV values calculated for 2 different concentrations of analytes in 40 different assays.
Bio-TechNE guarantees an in-batch CV of 10% and a maximum of 5 through strict QC standards6%；The index of more than 95% of the inter-batch CV value is controlled within 15% to ensure the performance of the test.
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