Aging is caused by a combination of internal and external factors. Intrinsic aging refers to age-dependent and natural aging, which is genetically determined.
At present, there are many anti-aging methods pursued by people to fight against aging, non-invasive, minimally invasive, surgical, non-surgical and so on. However, these** usually have a longer recovery time and are prone to pigmentation and scarring.
At present, with the increasing prominence of aging problems, the related research of aging and anti-aging has become the focus of medical research.
In recent years, there have been more and more articles on aging and anti-aging in top medical professional journals such as Science, Nature, and Cell.
Mesenchymal stem cells exert anti-aging effects
Umbilical cord mesenchymal stem cell (MSCS) supernatant purification exosome microneedle injection is a safe and effective method of facial rejuvenation**, which is expected to become a new method of anti-aging.
The stem cell supernatant is rich in a variety of cytokines that can activate cells in damaged parts of the body and forcibly restore tissue function to aging phenomena. (Stem cell medium, also known as supernatant.) The stem cell supernatant (culture medium) is rich in various nutrients, which have a nourishing effect on stem cells, and it can make stem cells live and proliferate healthily).
Mesenchymal stem cells (MSCs) are popular in the field of rejuvenation due to their ease of isolation, proliferation, and pluripotency. They release a variety of growth factors in both autocrine and paracrine ways.
A recent study showed that umbilical cord mesenchymal stem cells UC-MSCS-conditioned supernatant (UC-MSCS-CM) can form immature adipocytes, type 1 collagen, and type 2 collagen compared to BMMSCS conditioned supernatant, thereby exerting anti-aging effects.
The team of Director Zhen Fenglin of Beijing Friendship Hospital affiliated to Capital Medical University recently published a randomized controlled clinical study in Frontiers in Medicine, aiming to evaluate the efficacy of human umbilical cord mesenchymal stem cell conditioned supernatant purification exosome (HUC-MSCS-CM) microneedle injection in brightening and rejuvenation.
Figure 1 A total of 28 volunteers with an average age of 41 years completed the study.
Clinical case of a tertiary hospital
The researchers' evaluation of brightness and texture, as well as self-satisfaction scores, showed that the umbilical cord mesenchymal stem cell conditioned supernatant purification exosome microneedle injection group was more effective than the microneedle (microneedle injection saline) group alone. No serious *** was reported throughout the study period.
The volunteers were divided into two groups, an umbilical cord mesenchymal stem cell supernatant purification exosome microneedling group and a saline microneedling group.
The face is anesthetized with a 5% compound lidocaine cream after cleansing. For one side, apply 10 ml of saline, and add the remaining 1. topically after microneedling treatment0 ml for a total of 192 needles with a length of 05 mm。Perform the same method on the other side. Participants received five **, each with an interval of 2 weeks, for a total of 10 weeks.
Concentration analysis of multiple cytokines secreted by human umbilical cord mesenchymal stem cells.
Representative images
The results showed significant improvements in brightness (reduction in melanin index, UV spots, and brown spots) and rejuvenation (reduction of wrinkles and pores, and increased elasticity) in the human umbilical cord mesenchymal stem cell group.
A a: ** anterior, b b: microneedle injection of HUC-MSCS-cm;
C c: ** before, d d: microneedle injection of normal saline;
b b: Shows greater improvement in brightness and pore size.
A a: ** anterior, b b: microneedle injection of HUC-MSCS-cm;
C c: ** before, d d: microneedle injection of normal saline;
b b: Periorbital wrinkles show greater improvement.
The researchers looked at 28 participants who were more satisfied with the overall satisfaction with the efficacy of the results without knowing (the participants were blindfolded and receiving microneedle injections).
Improve metrics
Melanin index: 2 weeks after the last **, the melanin decreases more, indicating the efficacy of exosomes for whitening (Figure 2a).
Ultraviolet spots: Ultraviolet spots are associated with exposure to ultraviolet solar radiation. A significant difference in the UV spot fraction on both sides was observed at the last measurement (Figure 2b).
Brown spots: 2 weeks after final**, there was a significant change in the brown spot score on either side with exosomes ** (Figure 2b).
Regeneration
Wrinkles:In the final measurement, there was a significant reduction (Figure 3a).
Pores:**The after-effect was significantly better than that of the microneedle side alone (Fig. 3b), indicating that stem cell exosomes can reduce pore size.
Elasticity:At 2 weeks after the last **, there was a significantly higher increase in the microneedle injection exosome group compared to the microneedle injection group alone (Figure 3b).
Exosomes are concentrated from the supernatant, which retains the active ingredients of the supernatant to the greatest extent and removes some protein molecules that are more likely to cause allergies, making the product more conducive to absorption.
The stem cell supernatant is rich in a variety of cytokines that activate cells in damaged parts of the body and forcibly restore tissue function to aging phenomena.
Anti-aging effects of exosomes
In June 2021, the team of Lee Ju Hee from the Institute of Biology of Yonsei University in Seoul, South Korea, published a study in the Journal of Cosmetic Diseases: Topical application of adipose mesenchymal stem cells (MSCs) combined with nicotinamide after laser has an anti-aging effect on **.
Twenty-five aging patients underwent partial laser ablation (AFL) on both sides of their faces to achieve rejuvenation.
The researchers applied stem cell supernatant plus 2% niacinamide to the designated side of the face of the patients in the experimental group and a moisturizer without these ingredients for three weeks.
The results showed that the wrinkle index, melanin index, patient satisfaction score and overall aesthetic improvement scale were significantly higher than those in the control group after using stem cell supernatant plus nicotinamide.