Basic information about SCCA1 SCCA2 recombinant protein:
1.Protein nomenclature: SCCA1 (Squamous Cell Carcinoma Antigen 1) SCCA2 (Squamous Cell Carcinoma Antigen 2).
2.Function: SCCA1 SCCA2 is a protein belonging to the Serpin (serine protease inhibitor) family that has the function of inhibiting protease activity.
3.Coding genes: SCCA1 The SCCA2 gene is located on different chromosomes of the human genome, respectively, the SCCA1 gene is located at 11q13Position 5, the SCCA2 gene is located at 5q333 Positions.
4.Protein structure: The SCCA1 SCCA2 protein molecule has about 390 amino acids and contains a signal peptide, an active pocket, a conserved anti-inhibitor domain, and a cell localization signal.
5.Physiological function: SCCA1 SCCA2 protein plays an important role in cells and is related to cell proliferation, angiogenesis and other processes.
1.Design primers: Design primers for SCCA1 and SCCA2 genes to ensure that the primers are highly matched to the target gene sequence.
2.Gene cloning: Amplify the SCCA1 SCCA2 gene by PCR and place it into a target expression vector, such as PET28A.
3.Vector transformation: Vector transformation of the target expression vector to E. coli (e.) is performed using chemical transformation or electroshockcoli).
4.Pre-culture: Colonies containing the expression vector of interest are inoculated into LB medium and pre-incubated at 37 °C.
5.Expressed protein: The resulting pre-culture solution is transferred into a medium containing IPTG to induce protein expression.
6.Cell harvesting: After protein expression has reached the appropriate level, cells are harvested by centrifugation.
7.Cell disruption: Cells are disrupted using ultrasound or other methods to release the protein of interest.
8.Stock collection: The supernatant of the broken cells is collected as a protein stock.
The experimental design enabled the preparation of SCCA1 and SCCA2 recombinant proteins through gene cloning, transformation, expression, and cell disruption. SCCA1 SCCA2 recombinant protein is a protein with important physiological functions, which can inhibit the activity of proteases. It is of great significance to grasp the basic information of SCCA1 and SCCA2 recombinant proteins and the experimental preparation methods to study their functions in important physiological processes such as cancer.