PBRM1 (Polybromo-1) is a protein closely related to chromatin remodeling and gene transcriptional regulation. It is an important histone binding protein, mainly found in the nucleus, by interacting with proteins such as histones, involved in the assembly and depolymerization of chromatin complexes in the nucleus, and by binding to transcription factors, regulating gene expression. The dysfunction of PBRM1 protein is closely related to the occurrence and development of a variety of diseases, including the occurrence and development of PBRM1 protein and the occurrence of other diseases. Catalog No. PA1000-8318
For the verification of the biological activity of PBRM1 protein, the following methods are mainly used:
1.Cell line experiments: The association between PBRM1 and these biological activities was verified by overexpressing or targeting the knockout of PBRM1 gene in the cell line to observe the changes in basic biological characteristics such as cell proliferation, apoptosis, and metastasis.
2.Transcriptional level analysis: The transcriptional regulation effect of PBRM1 on specific genes was detected by real-time quantitative PCR and other methods to verify its role in transcriptional regulation.
3.Protein-protein interaction analysis: The interaction between PBRM1 and other proteins was confirmed by immunoprecipitation and fluorescence co-localization, and its protein complexes were identified, and its functional mechanism was further studied.
The structural functions of PBRM1 protein mainly include the following aspects:
1.The PBRM1 protein contains multiple -N-acetyllysine residues that can be combined by acetylation modifications of histones and mediate gene transcriptional regulation within the nucleus.
2.PBRM1 protein contains multiple domains, including the Bromo domain, HMG domain, and SWI SNF domain, which are closely related to processes such as chromatin remodeling, gene expression, and cell proliferation.
3.PBRM1 protein is involved in the regulation of important biological processes such as apoptosis and angiogenesis by binding to other proteins such as p53 and HIF-1.
In general, PBRM1 protein, as an important histone binding protein, plays an important role in chromatin remodeling and gene expression regulation. Through the verification of its biological activity and the study of its structure and function, it can provide an important reference for further revealing its mechanism of action in the occurrence and development of diseases, and also provide a theoretical basis for the development of related drug targets.