DPAGT1 recombinant protein is a protein encoded by the DPAGT1 gene, which is mainly used to study the biological function of protein glycosylation and the mechanism of related diseases. DPAGT1 protein is a substrate-specific migration enzyme involved in the core steps of the N-glycosylation pathway. The methods for verifying its biological activity mainly include the following:
Catalog No. PA1000-8320
1.Substrate-specific assays: Substrate specificity is verified by measuring the transfer activity of DPAGT1 protein to specific substrates. Commonly used methods include in vitro substrate transfer activity assays and experiments based on chemically synthesized substrates.
2.Animal model validation: The role of DPAGT1 in physiological and pathological processes was verified by establishing in vivo animal models of DPAGT1 gene knockout or overexpression and observing its biological performance.
3.Molecular interaction verification: Protein-protein interaction experiments were conducted to verify the binding ability of DPAGT1 to other proteins and reveal its mechanism of action in the signaling pathway.
The structure and function of DPAGT1 protein mainly include the following aspects:
1.Structural characteristics: DPAGT1 protein contains a long N-terminal extracellular domain and a short C-terminal intracellular domain. Its N-terminal extracellular domain contains three N-glycosylation sites for substrate binding and substrate transfer. The C-terminal intracellular domain is mainly involved in the catalytic reaction of substrate transfer.
2.Catalytic mechanism: DPAGT1 protein completes substrate transfer through substrate-specific selection and glycosylation. In the N-terminal extracellular domain, the substrate binds to the DPAGT1 protein to form a substrate-enzyme complex, and the N-acetylglucosamine residue on the substrate molecule is transferred to the substrate molecule.
3.Functional regulation: The activity of DPAGT1 protein is affected by substrate concentration, glycosylation pathway, and cofactors. These factors directly affect the role and biological function of DPAGT1 protein in the glycosylation pathway.
Overall, DPAGT1 recombinant protein is an important research tool, which can reveal its mechanism of action in the glycosylation pathway through the verification of biological activity and the study of structure and function, and provide important information for related diseases and drug development.