Detection of thrombin activity is critical in biomedical research, especially in rat models. Thrombin plays a key role in blood clotting, so accurate measurement of its activity is invaluable for understanding disease pathogenesis, evaluating drug effects, and conducting relevant biomarker studies. Now, an ELISA kit specifically for the detection of thrombin activity in rats has come into being, bringing good news to the majority of scientific researchers. The rat thrombin activity ELISA kit is used for the quantitative detection of thrombin content in serum, plasma, tissue homogenates and related liquid samples in vitro.
The Rat Thrombin Activity ELISA Kit is based on enzyme-linked immunosorbent assay (ELISA), which uses the specificity of the antigen-antibody reaction to achieve accurate detection of thrombin activity in rats. Compared with traditional methods, ELISA has higher sensitivity and specificity, and can more accurately reflect the actual activity of thrombin.
Ready-to-use: Each kit consists of antibody-coated plates and ready-to-use reagents that require no special handling and are simple to use.
Rat thrombin Assay Kit Rat thrombin kit assay procedure.
1.Add 50ul of standard sample diluent to the blank well, add 50ul of standard or sample to be tested in each of the remaining wells, mix the reaction plate and leave it for 37 or 40 minutes.
2.Wash the plate with 1 wash solution 4-6 times, add 1 wash solution 350ul per well, soak for 1-2 minutes each time, and print dry on the filter paper.
3.100ul of biotinylated antibody diluent was added to the blank well, and 100ul of 1* biotinylated antibody working solution was added to each of the remaining wells, and mixed well for 37 and 30 minutes.
4.Wash the plate as above.
5.Add 10oul of SABC complex working solution to each well, mix well and leave for 37 for 20 minutes.
6.Wash the plate as above.
7.Add 100ul of pre-configured TMB mixture to each well, mix well, and then set it for 37 dark places to react for 10-20 minutes (the specific color development time depends on the color development results).
8.Add 100ul stop solution to each well, mix well, and measure the absorbance value at 450 nm with a microplate reader within 30 minutes.
Rat thrombin Assay Kit Rat Thrombin Kit Reagent Preparation:
1. Assay plate: one piece (96 or 48 wells).
2. Standard: 2 bottles (freeze-dried).
3. Sample diluent: 1 20ml bottle.
4. Biotin-antibody diluent: 1 10ml bottle.
5. Horseradish peroxidase-labeled avidin dilution (HRP-**idin diluent): 1 10ml bottle.
6. Biotin-antibody: 1 120 L bottle (1:100).
7. Horseradish peroxidase labeled avidin (HRP-**IDIN): 1 120 L bottle (1:100).
8. Substrate solution (TMB substrate): 1 10ml bottle.
9. Wash buffer: 1 20ml bottle, each bottle is diluted with distilled water 25 times when used.
10. Stop solution: 1 10ml bottle (2N H2SO4).
The Rat Thrombin Active ELISA Kit has become a powerful assistant in biomedical research due to its excellent performance and wide range of applications. Whether it is a scientific research institution or a university, it is worth having such a high-quality kit to bring more possibilities to your scientific research work.