swath"Yes"sequential window acquisition of all theoretical mass spectra"It is a mass spectrometry technique that is commonly used for proteomics quantification. Today, let's take a look at the basic principles and characteristics of SWATH quantitative proteomics:
Figure 1Swath Quantitative Proteomics Service Technology Platform.
1. Basic principle:
In a SWATH analysis, the mass spectrometer continuously and automatically scans a series of mass windows, covering all potential precursor ions. In this way, theoretically all possible ions will be detected. Compared to qualitative data-dependent scanning (DDA), SWATH provides a more systematic and unbiased approach to data collection for data-independent scanning (DIA).
2. Quantitative characteristics:
SWATH is characterized by its ability to perform high-throughput, high-repeatability, and high-precision quantification of multiple components in complex samples. This makes it particularly suitable for studies that require large-scale sample cohort comparisons, such as biomarker discovery.
3. Data processing
Because the data collected by SWATH is very complex, it often requires specific software and algorithms for data extraction and quantification. These software use a library of pre-acquired mass spectra of protein peptides to aid identification and quantification.
4. Application field:
SWATH mass spectrometry technology has applications in a variety of biological research, including biomarker identification, pathway analysis, protein-protein interaction studies, and more.
5. Comparison with other technologies:
Compared to traditional data-dependent scanning (DDA) techniques, SWATH provides better quantitative repeatability and a wider dynamic range. But due to its data complexity, data analysis can be more challenging.