NADH (reduced coenzyme I) and NADPH (reduced coenzyme II) are important coenzymes in living organisms, and they play different roles in cellular metabolism.
Mode of production: NADH is mainly produced by respiration, while NADPH is mainly produced in the photoreaction phase of photosynthesis.
Site of action: NADH is mainly used in the citric acid cycle in glycolysis and cellular respiration, while NADPH is mainly produced in the pentose phosphate pathway and is mainly used for the synthesis of nucleic acids and fatty acids.
Physiological functions: NADH is mainly involved in material energy metabolism, while NADPH is mainly involved in anabolism and antioxidant.
Detection method: Due to the similar fluorescence spectra of NADH and NADPH, it is difficult to distinguish between these two small molecules by traditional autofluorescence analysis methods. The research team of Prof. Yi Yang and Prof. Yuzheng Zhao from the State Key Laboratory of Bioreactor Engineering (East China University of Science and Technology) collaborated with Prof. Haiyan Liu from the University of Science and Technology of China to develop a series of high-performance genetically encoded fluorescent probes INAP for the specific detection of Nadph through the rational design and modification of substrate-binding proteins, realizing high-temporal and spatial resolution detection and imaging of Nadph metabolism in living organisms, living cells and various subcellular structures.
In conclusion, although there is only one word difference between NADH and NADPH, there are significant differences in their functions and mechanisms of action in living organisms. For more information, it is recommended to consult a professional biology book or consult a biology expert.