I. Introduction.
Pig artificial insemination technology is an important means to improve reproductive efficiency and pig utilization rate in modern pig industry. In the process of artificial insemination, the preservation of ** is one of the key links. This article will introduce in detail the storage methods and precautions of pig ** for reference.
Second, the importance of pig preservation.
The preservation of pig ** is not only related to the success rate of fertilization, but also directly affects the quality of embryos and the health of piglets born. High quality is the basis for good reproductive performance, and the right storage methods ensure that quality is not compromised.
Third, the pig ** preservation method.
Store at room temperature: 15-25 room temperature, fresh** can be stored for 2-4 hours. In order to keep the temperature stable, you can keep it in a thermos flask or incubator.
Cryopreservation: Store ** at a low temperature of 4-10 to prolong its storage time. This method is usually used for short-term storage and can maintain **activity for up to 1-2 days.
Cryopreservation: The long-term storage of pigs** is usually carried out by the freezing method. By lowering the temperature to -196, it can be stored for months or even years. During the freezing process, appropriate cryoprotectants need to be used to reduce the damage of ice crystals to sperm.
Fourth, the pig ** preservation precautions.
Temperature control: Regardless of the storage method, temperature stability is crucial. Temperatures that are too high or too low can affect sperm motility and fertilization. Therefore, it should be monitored regularly and ensure that the temperature is within the appropriate range.
Container selection: The container used for preservation** should have good thermal insulation properties to reduce temperature fluctuations. At the same time, the container should be kept clean to avoid contamination.
Shockproof and shockproof: In the process of storage and transportation, the impact of vibration on the ** should be avoided as much as possible. Vibrations can cause sperm damage, which can reduce fertilization rates. Therefore, appropriate shock absorption measures should be taken.
Avoid frequent freeze-thaw cycles: During cryopreservation, frequent freeze-thaw should be avoided. Each freeze-thaw will cause some damage to the sperm, thus reducing its ability to fertilize. Therefore, the number of freeze-thaw cycles should be minimized and reliable cryoprotectants should be selected.
Regular inspection: During the preservation process, the quality and vitality of ** should be checked regularly. If it is found that the quality of ** is declining or the vitality is insufficient, measures should be taken in time to avoid losses.
Use of qualified**: Pigs used for artificial insemination should come from healthy breeding pigs and have undergone rigorous inspection and selection. Unqualified or unhealthy** not only affects fertilization success rates, but can also spread diseases.
Personnel training: Personnel who carry out pig ** preservation should receive professional training to understand relevant knowledge and skills. Correct operation and precautions ensure that the quality is not compromised and the fertilization success rate is increased.
Monitor the environment: During storage, you should pay close attention to environmental conditions, such as temperature, humidity, etc. If an abnormal situation is found, measures should be taken to adjust it in time to ensure that the environmental conditions are suitable for preservation.
In short, the preservation of pig ** is an important link in the process of artificial insemination. In order to ensure the success rate and embryo quality, it is essential to take appropriate preservation methods and strictly follow the relevant precautions. Proper operation and management can lead to higher profitability and performance in the pig industry.