Leptospira physical examination test kit
The kit is used for the qualitative detection of leptospira antibodies in serum and plasma
Clinical manifestations of leptospira range from mild typhoid fever to jaundice with severe liver and kidney damage.
Compilation: Shenzhen Henghao Biotechnology***
Hook bodies are found in rodents and most domestic animals, parasitize in the renal tubules, and are then excreted in the urine.
Human infection** occurs from direct contact with infected animals (e.g. veterinarians, slaughterhouse workers, or milk).product workers) or air contaminated by animal carriers (e.g., agricultural workers), and water exposed to water used for bathing or swimming by animals that have been shown to be potentially at risk of infection.
The hook body enters the host cell through the broken **, mucosal surface or eye, and the incubation period ranges from 3 to 30 days, but it is usually found at 10-12 days, and antibodies can be detected between the 6th and 10th days of the disease, and the peak is reached within 3-4 weeks, after which the antibody level will slowly decrease, but the antibody can still be detected after several years.
DetectionPrinciple].
The microplate has been coated with purified leptospira antigen, the first incubation, the antibody in the sample serum binds to the antigen on the microplate, the unbound sample is washed away, the enzyme conjugate is added, and the antibody bound in the well is conjugated, after the second wash, the TMB substrate solution is added, the color turns blue, the stop solution is added, and the result can be read directly or detected by the microplate reader.
Detection method
Equilibrate all reagents and samples to room temperature before the experiment.
During the test, avoid the formation of air bubbles in the wells, which can affect the entire experiment and the final result reading, and pat the residual liquid on absorbent paper after washing.
Negative and positive controls are diluted and no further dilution is required.
Do not add RF sorbent to negative and positive control.
Detection steps
1.Secure the slats required for the experiment to the plate rack (two controls plus sample).
2.Sample serum is diluted with dilution buffer in a 1:40 ratio (10 l serum + 390 l of dilution buffer).
Prepare the required number of sample dilution tubes. Add 100 l of the diluted sample to the corresponding tube. 40 L of RE adsorbent was added to each microwell with samples, mixed well, and incubated at room temperature for 5-10min. This step cannot be performed in microwells.