Brief introduction.
TMB is catalyzed by HRPase and converted to its final yellow color by the action of acid. There is a positive correlation between the shade of color and the interleukins in the sample. The absorbance (OD value) was determined with a microplate reader at a wavelength of 450 nm, and the human interleukin content in the sample was calculated by a standard curve.
Interleukin 1 specificity: HU IL-1A, detection range: 39pg ml 250pg ml, sensitivity: 2pg ml, specimen dosage: 100ul well. This kit is prepared using the principle of dual-antibody sandwich ELISA method, which is suitable for the quantitative detection of native and recombinant IL-1A concentrations in human serum, plasma, tissue, body fluid, or cell culture supernatant in vitro. Interleukin-1 (IL-1), a collective term for two proteins, IL-1 and IL-1, is genetically encoded, but they recognize the same receptor on the cell surface.
IL-1 and IL-1 are structurally similar, with 25% homology at the amino acid level; Both are cleaved from a molecular weight 31 kda precursor to 175kda of protein. Neither IL-1 nor IL-1 contains typical hydrophobic signal peptides, but there is evidence that some atypical pathways can secrete these factors. A large amount of IL-1 precursor form is retained intracellularly. A fraction of the unmodified IL-1 is transported to the cell surface but remains attached to the cell. Unmodified membrane-bound IL-1 exhibits biological activity, and adjacent paracellular IL-1 is secreted. IL-1 and IL-1 exert their effects when they bind to specific receptors.
Specificity: Human IL-2, Detection Range: 78pg ml 500pg ml, high sensitivity: 4pg ml, high specificity: does not react with IL-1, 3, 4, 5, 6, 8, 10, 12, ifn, TNF, vegf, TGF and ICAM, etc., high repeatability: the coefficient of variation within and between plates is 10%, and the specimen dosage: 100ul well.
Interleukin 2 This experiment uses a double-antibody sandwich ELISA method, which is suitable for the quantitative detection of native and recombinant IL-2 concentrations in human serum, plasma, tissue or cell culture supernatants in vitro. Interleukin-2 (IL-2) is predominantly expressed by mitogen or antigen-activated T lymphocytes. It plays an important role in promoting antigen-specific proliferation of T cell clonal proliferation. In addition, IL-2 is also involved in multiple immune responses in other different types of cells.
IL-2 stimulates thymocyte proliferation; stimulates the proliferation and differentiation of activated B cells; Promote the growth, differentiation and cell killing ability of monocytes; induce the growth of natural killer cells and stimulate the cytokine expression and cell killing ability of these cells; Increases the production of lymphatic activating killer cells (LAKs); Induce the proliferation and differentiation of oligodendrocytes. The cDNA of human IL-2 encodes a 153-amino acid glycoprotein precursor containing a 20-amino acid signal peptide that is cleaved off during maturation. At the amino acid level, the sequence homology of mouse IL-2 and human IL-2 was 60%. Human IL-2 can be activated in mouse cells, but mouse IL-2 cannot be activated in human cells. The IL-2 gene is mapped to the 4q locus of chromosome.
Related series of products
Human interleukin-4 (IL-4) ELISA kit, human interleukin-5 (IL-5) ELISA kit, human interleukin-6 (IL-6) ELISA kit, human interleukin-8 (IL-8) ELISA kit, human interleukin-10 (IL-10) ELISA kit, human interleukin-13 (IL-13) ELISA kit, human interleukin-17 (IL-17) ELISA kit, human interleukin-18 (IL-18) ELISA kit, human interleukin-20 (IL-20) ELISA kit。