Strain cultivation technology refers to the process of cultivating the target strain in a suitable culture medium to provide the necessary nutrients and conditions under the control of environmental conditions and the cultivation process, and to accelerate the growth of mycelium, spore formation and reproduction. This article will introduce the basic process and main steps of culture cultivation in detail.
1. Strain selection and culture medium.
Strain selection is the first step in strain planting, key words: strain selection, characteristic analysis.
1.According to the research purpose and needs, select the appropriate strain for research and culture.
2.For the selected strains, the characteristics were analyzed, including morphological characteristics, growth Xi, metabolic pathways, etc. This can help determine the appropriate culture environment and medium.
2. Culture environment and media conditions.
The culture environment and medium conditions will directly affect the growth and reproduction of strains, keywords: environmental conditions, medium selection.
1.The control of environmental conditions such as temperature, humidity, light and ventilation has an important impact on the growth of cultures.
2.Choosing the right medium is the basis for culturing cultures. Commonly used media include agar, sucrose agar, soy protein agar, etc. The addition of carbon sources, nitrogen sources, trace elements, and buffers to the culture medium needs to be adjusted according to the preferences and needs of the culture.
3. Meristem technology.
Strain meristem technology aims to promote hyphal growth, spore formation and reproduction, keywords: meristem technology, hyphal growth, spore formation.
1.Mycelial growth technology is to promote the growth and diffusion of mycelium by selecting appropriate culture conditions and media to provide nutrients and environmental conditions required for mycelial growth.
2.Spore formation technology is to promote the formation of spores by adjusting the conditions such as culture temperature, humidity and light when the strain reaches a certain growth period.
3.Spore propagation technology refers to the process of inoculating spores into a new medium to create suitable environmental conditions that allow the spores to germinate into new hyphae.
4. Strain storage and preservation.
Strain storage and preservation is an important part of ensuring the quality and long-term preservation of strains.
1.Commonly used strain storage techniques include refrigeration, lyophilization, liquid nitrogen freezing, etc. According to the characteristics of the strain and the different shelf life, choose the appropriate storage method.
2.The storage conditions of the strains need to pay attention to the control of factors such as temperature, humidity, dryness and dark light to ensure the survival rate and quality of the strains.
Conclusion: Strain cultivation involves many aspects, such as strain selection, medium conditions, culture environment, meristem technology, and strain storage and preservation. Only by grasping these key points and rationally regulating them can we achieve rapid reproduction and effective utilization of strains. At the same time, strain cultivation is a complex process, which requires experienced personnel to guide the operation, be sure to comply with relevant laboratory safety regulations, and strengthen the research and application of strain cultivation technology.
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