Chinese name: dibenzocyclooctyne-isothiocyanylfluorescein
Chinese aliases: dibenzocyclooctyne modified fluorescein.
Product Name:dbco-fitc
Synonyms: fluorescein-dbco
CAS Number: 2054339-00-1
Molecular weight 66571
Molecular formula C39H27N3O6S
Uses: Only for scientific research, not for human body.
dbco-fitc diphenylcyclooctene-fluorescein is a commonly used biofluorescent labeling reagent, which is widely used for the labeling of proteins, peptides and other biological macromolecules. It works by taking advantage of the nucleophilic reactivity of diphenylcyclooctene (DBCO) to perform copper-free click chemistry with biomolecules containing amino groups to generate stable covalent bonds. At the same time, the presence of fluorescein (FITC) allows labeled biomolecules to fluoresce, making them easy to observe and track under the microscope.
DBCO-FITC diphenylcyclooctene-fluorescein has a wide range of applications, especially in the biomedical field. For example, in cell-based experiments, scientists can use dbco-fitc to label specific proteins or antibodies, and then observe the distribution and dynamics of these proteins within the cell. In addition, it can also be used for in vivo fluorescence imaging to help scientists study the mechanism of action of drugs in living organisms and the occurrence and development of diseases.