DSPE-PEG is synthesized from dipalmitoylphosphatidylethanolamine (DSPE) and polyethylene glycol (PEG) by esterification. DSPE is a type of phospholipid that has good biocompatibility and stability, whereas PEG is a polymer that has good water solubility and biocompatibility. Through esterification, DSPE and PEG are linked together to form a bioactive material with good biocompatibility and stability.
The T7 targeting peptide is a short peptide consisting of 7 amino acids with high affinity and the ability to specifically bind to tumor cell surface receptors. The synthesis of T7-targeting peptides mainly uses solid-phase synthesis, which links the required amino acids together in predetermined sequences through multi-step chemical reactions, and finally gives T7-targeting peptides.
The ligation of the DSPE-PEG and T7-targeting peptides is primarily an amide reaction. The carboxyl group of dspe-peg is linked to the amino group of the T7-targeting peptide to form an amide bond, resulting in the dspe-peg-t7 targeting peptide.
Structural features. 1.The linkage between DSPE and PEG makes the whole molecule have good water solubility and stability, which is conducive to the delivery and release of drugs.
2.The introduction of T7-targeting peptide enables the whole molecule to have high affinity and specificity, and can bind to tumor cell surface receptors to achieve targeted drug delivery.
3.The structure of DSPE-PEG-T7 targeting peptide has a certain flexibility, which can adapt to different physiological environments and improve the bioavailability and effect of drugs.
Targeting capabilities. 1.Binding to tumor cell surface receptors: T7 targeting peptides have the ability to bind to tumor cell surface receptors with high affinity and specificity, enabling targeted drug delivery.
2.Drug release ability: The DSPE-PEG-T7 targeting peptide can be endocytosed into cells under the action of tumor cell surface receptors, thereby achieving intracellular drug release.
3.Low toxicity to normal tissues: Due to the structural characteristics and specificity of the DSPE-PEG-T7 targeting peptide, it has low toxicity to normal tissues, resulting in an improved safety profile.
Characteristics of the application direction.
1.In the field of drug delivery: DSPE-PEG-T7 targeting peptide can be used as a drug carrier to deliver drugs into tumor cells in a targeted manner, thereby improving the efficacy of drugs and reducing the efficacy of drugs At the same time, due to its good biocompatibility and stability, it can prolong the circulation time of drugs in the body and improve the bioavailability of drugs.
2.In the field of genes**: DSPE-PEG-T7 targeting peptide can be used as a carrier of gene vectors to deliver genes into tumor cells, so as to achieve genes for tumor cells**. At the same time, due to its good biocompatibility and stability, it can protect genes from the degradation of enzymes in the body and improve genes.
The ** effect.
3.In the field of cell imaging: DSPE-PEG-T7 targeting peptide can be used as a cell imaging agent to achieve imaging and localization of tumor cells by binding to tumor cell surface receptors. At the same time, due to its good biocompatibility and stability, it can prolong its circulation time in vivo and improve the imaging effect and accuracy.