In biochemistry and molecular biology research, buffers are indispensable experimental reagents to maintain the stability of the experimental system. N-(2-hydroxyethyl)piperazine-N'-3-Propanesulfonic acid (EPPS) is a commonly used buffer with an effective pH range of 73-8.7, pka80(25), by preparing EPPS buffer, the environment can be maintained in a certain pH range, so as to ensure the accuracy of the experimental results. So, how is it formulated?
Preparation steps for EPPS buffer:
1. Preparation 01 M EPPS solution (solution A):
1) Weigh about 25233 g of EPPS powder and place in a clean volumetric flask.
2) Add an appropriate amount of deionized water and set the solution volume to 1000 ml (1 liter).
3) Stir thoroughly until the EPPS powder is completely dissolved.
2. Preparation 01 M NaOH solution (solution B):
1) Weigh about 4 grams of NaOH powder and place it in a clean volumetric flask.
2) Add an appropriate amount of deionized water and set the volume of the solution to 1000 ml.
3) Stir well until the NaOH powder is completely dissolved.
3. Mix EPPS and NaOH solution
The prepared 01 M EPPS solution (solution A) and 01 M NaOH solution (solution B) is mixed together in the desired volume ratio. Stir well during the mixing process to ensure that the two solutions are fully mixed and even.
4. Adjust pH value
Add the NaOH solution dropwise to the mixed solution while measuring the pH of the solution with a pH meter to bring it to the desired range with a pH of EPPS buffer at 73-8.7 between.
The above steps can be used to successfully prepare EPPS buffers for use in various biochemical experiments. Take care to use precise weighing tools during the preparation process to ensure that the concentration and pH of the solution are accurate. At the same time, it is completed in strict accordance with the experimental procedures and follows the safe operation, which can fully ensure the accuracy of the experimental results.