Ambient air cleanliness testing method.
Detection conditions. 1.Static detection.
The test was carried out when the environmental purification and air-conditioning system in the laboratory animal facility was in normal continuous operation for more than 48 hours, the process equipment had been installed, and there were no animals and staff in the room.
2.Dynamic detection.
Testing is carried out in a laboratory animal facility under normal production or experimental working conditions.
Testing instruments. 2.1 dust particle counter.
2.2. Measuring instruments should be checked regularly.
3 .Assay method.
3.1. Static detection.
3. 1 .1. The clean area and its purification and air-conditioning system should be thoroughly cleaned.
3. 1 .2. The measuring instrument is fully preheated, the sampling tube must be clean, and leakage is strictly prohibited at the connection.
3. 1 .3. The length of the sampling tube should be the allowable length of the instrument, and when there is no provision, it should not be 15 m。
3. 1 .4. The flow velocity of the sampling nozzle should be close to the average wind velocity of the clean room section. The inspector should be on the downwind side of the sampling port.
3.2. Dynamic detection.
In the clean laboratory animal facility, the laboratory animal breeding room and the animal laboratory area, select the upwind direction of the air flow of the representative measurement point for testing, and the operation rules of the detection method are the same as those for static testing.
4. Measurement point arrangement.
4.1. When testing the clean experimental work area, if there is no special experimental requirement, the sampling height is 1 from the ground0 m high on a work plane.
4.2. When testing the clean laboratory animal room, the sampling height is the ** horizontal height of the cage height, which is about 091.0 m on a flat surface.
4.3. The spacing between the measurement points is 052.0 m laminar flow 2 cleanroom measurement points total 20 points. If the turbulent clean room area is not more than 50 m2, 5 measuring points should be arranged, and 3 5 measuring points should be added for every 20 50 m. Each measuring point was measured 3 times in a row. e5 Sampling flow rate and sampling volume.
5.Class 1 100 requires a sampling flow rate of 1 for clean laboratory animal facilitiesThe sampling volume of 0 l min is not less than 10 l。
5.2. The sampling flow rate of clean laboratory animal facilities required by 1000 or above is 0The sampling volume of 5 l min is not less than 10 l。e6 result calculation.
6.1. Each measurement point should be continuously sampled and measured 3 times under the condition of stable operation of the test instrument, and the average value should be calculated and calculated as the measured result of the point.
6.2 for greater than or equal to 0The number of dust particles at 5 m determines the maximum value of each measurement point in the laminar flow cleanroom. The turbulent clean room takes the average value of each measurement point as the measured result.