The automated oocyte voltage clamp system is a fully automated toad oocyte expression system

Mondo Science Updated on 2024-02-01

Automatic oocyte voltage clamp systemIt is a fully automated toad oocyte expression system that uses dual-electrode voltage-clamp technology TEVC for moderate-throughput screening of ligand-gated and voltage-gated ion channels and electro-producing transporters.

Automatic oocyte voltage clamp systemThe automation of TEVC records has transformed drug discovery:

Much higher throughput can be achieved at a much lower cost.

The general conditions of the experiment can be standardized, thus improving reproducibility.

It frees your highly qualified personnel from routine tasks and allows them to focus on experimental design and data analysis.

It can be run overnight without supervision. Users can start an additional experiment at the end of the workday and analyze the results the next morning.

Designed for humans with Xenopus laevis oocyte expression system.

The automated oocyte voltage clamp system is suitableStandard 96-well plates were recorded. Oocytes are seeded into the wells within a few minutes. They quickly settle in conical wells and adhere to the bottom of the well after a few hours. The oocytes no longer need to leave the plate; You can easily transfer oocytes from the incubator to the device and back again.

The well plate bracket is driven by a linear motor and hovers smoothly and noiselessly on a compressed air cushion above the magnetic steel plate. It has an operating resolution of 20 m. Aside from occasional cleaning of the steel plates, the entire system does not require any maintenance.

The vertically moving Z-arm fixed TEVC probe is designed to meet the high demands on speed and precision. The z-arm moves with a resolution of 20 m; The position and speed are controlled by a computer. The rapid regulation process guarantees precise piercing of the oocytes. We recommend using off-the-shelf sensors supplied with multi-channel systems. If you prefer to produce your own, we highly recommend the blank measuring head from the MCS. You can then attach your own capillaries, priming tubes, and silver wires to the mounting bracket.

Automatic oocyte voltage clamp systemThe package includes everything you need to start up right away, including a high-performance computer with a display, TEVC probes, tubing, RoboFlow liquid handlers and accessories.

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