Betaine was originally extracted from sugar beets and is one of the plants that contains the most betaine. It is widely distributed in animals, plants, and microorganisms, and silently plays an important metabolic role. According to the relevant literature, betaine can promote fat metabolism, protect the kidneys, increase appetite and other effects.
Why it can promote fat metabolism shows that betaine must play an important regulatory role in the circulation process of fat metabolism. Then, detecting the content of betaine, understanding the molecular mechanism of betaine, and studying the metabolic process where betaine is located are not only conducive to the application of betaine function, but also can play a regulatory role in human health. At the same time, betaine also has the effect of dilating blood vessels. Understanding why it works the way it does is something that needs to be studied in depth.
If the betaine content is to be detected, the betaine extract needs to be separated from the sample before it can be determined on a chromatograph. Here, again, we use high-performance liquid chromatography. How to extract betaine still depends on its chemical or biological properties. It is dissolved in water or methanol and ethanol, and betaine has an aromatic sweet taste, so it can be extracted by water or alcohol.
High performance liquid chromatography (HPLC) can quickly and accurately detect the content of betaine. In this case, a reversed-phase C18 column was used for the detection of betaine content, and the response data was obtained with a UV detector at a wavelength of 254 nm. The content of betaine in the sample was determined by external standard method. The betaine standard stock solution was diluted with acetonitrile solution, and the samples were detected from low to high in turn, the mass concentration was the abscissa, and the corresponding peak area was used as the ordinate for linear regression, and the standard curve regression equation was obtained. The standard working solution and the sample volume solution were injected for determination, and the chromatographic peak retention time was used to qualitatively quantify, and the chromatographic peak area of the standard sample and the peak area of the sample solution were compared and quantified.