The Agilent 1200 Series LC System is an Agilent Technologies LC system comprised of a series of modular components that cover a wide range of needs, from routine analysis to complex research applications, for a wide range of laboratories, including pharmaceutical, environmental monitoring, food safety, biotechnology, and more.
Drug R&D and quality control
In the drug development process, it is used for the quantitative analysis of components, impurities and metabolites;In the quality control of the pharmaceutical production process, it is used to ensure that the quality of APIs and preparations meets the standards.
Food testing
Qualitative and quantitative analysis of additives (e.g., preservatives, pigments), contaminants (e.g., pesticide residues, heavy metals), nutrients (e.g., vitamins, fatty acids) and illegal additives in food.
Environmental monitoring
Detect contaminants such as POPS, polycyclic aromatic hydrocarbons (PAHs), and more in water, soil, and atmospheric samples.
Biological Sciences and Clinical Research
It is used for protein purity identification, peptide mapping in proteomics research;and the analysis of small molecule metabolites in biological samples (e.g., blood, urine, tissues).
Chemical industry
Monitoring and product quality control of the synthesis process of various chemicals, fine chemical products and new materials.
Forensic science
It is used for the analysis of toxicological samples, such as the detection of alcohol, metabolites, etc.
Quaternary low-pressure gradient pump
This module is the heart of the entire LC system, delivering the mobile phase and enabling gradient elution of the solvent. Provides accurate and stable flow control to support isocratic to complex multi-solvent gradient analysis.
Model Number:e.g. G1311a G1312A
Flow Rate Range:0.001 ml min to 5 ml min or higher (depending on model and configuration).
Pressure Range:up to 600 bar
Accuracy:Flow accuracy is typically better than 01% rsd
Solvent selection and switching time:Fast solvent conversion capability, gradient elution procedure.
Autosampler
Autosamplers are used to quickly and accurately inject samples into liquid chromatography systems, reducing manual handling errors and improving productivity and data quality. It typically has multiple volume setting options, supports microliter to milliliter sample volumes, and has good repetition and low cross-contamination rates.
Model Number:Such as G1313A
Tray capacity:Holds hundreds of vials, the exact number of which varies by model.
Injection volume range:Generally at 0Adjustable from 1 l to 1000 l.
Injection precision and repeatability:High-precision syringe pump to ensure low cross-contamination rate and good repetition.
Temperature control:Some models support cooling to maintain the stability of biological samples.
Column Heater:
Column heaters provide accurate control of the operating temperature of the column, which is critical for optimizing separation conditions and maintaining the stability and reproducibility of analytical results. The temperature range is generally adjustable from 4 to 80 with an accuracy of 01℃。
Model Number:Such as G1316A
Temperature Range:4 to 80
Temperature Stability and Accuracy:
Column capacity:Accommodates a wide range of I.D. and column lengths.
UV-Vis light detector:
This is a commonly used detector for qualitative and quantitative analysis by measuring the absorption of a sample component by a specific wavelength of ultraviolet or visible light. It has a wide linear dynamic range and high sensitivity.
Model Number:Such as G1315B, etc.
Wavelength range:Typically 190 nm to 700 nm or wider.
Sensitivity:The min detection limit is typically less than 1 x 10 -5 aufs
Linear Dynamic Range:>3 AUFS
Bandwidth:It can be adjusted to optimize signal resolution and speed as needed.
Diode Array Detector:
In contrast to conventional UV detectors, diode array detectors are able to acquire complete spectral data in a single run, enabling simultaneous multi-component analysis and structural information acquisition.
Model Number:Such as G1315D
Wavelength Sweep Range:Similar to UV-Vis light detectors.
Data Acquisition:Real-time full-spectrum scanning.
Sample Rate:High-speed data acquisition of multiple points per second.
Evaporative Light Scattering Detector:
ELSD is mainly used for the detection of non-ultraviolet absorbent samples that do not contain chromophores or fluorophores but have volatile, especially suitable for the detection of sugars, lipids and some polymer compounds.
Other Interface Modules:
For example, the electrospray ion source (ESI) and atmospheric pressure chemical ionization source (APCI) interfaces connected to the mass spectrometer enable the liquid chromatography system to be coupled with mass spectrometry technology for more in-depth sample characterization and structural identification.
Preparation before operation
1.Prior to the experiment, ensure that all modules are properly installed and connected, warmed up and initialized according to the manufacturer's guidelines.
Solvent & Sample Handling
1.Make sure that the mobile phase used is suitable for the LC system and that it has been degassed.
2.Samples should be disposed of properly to avoid contamination or damage to the system. For autosamplers, the vials need to be configured and loaded correctly.
System parameter setting
1.Set parameters such as flow rate, pressure, gradient program, etc., according to the experimental requirements.
2.For the detector, select the appropriate wavelength and response mode.
Security measures
1.Comply with laboratory safety regulations, including, but not limited to, electrical grounding, explosion protection, and solvent handling.
2.Do not open the pump head or other sealing parts at high pressure.
Data recording and storage
1.Regularly back up and properly store analysis results for later validation and audits.
Daily cleaning
1.Clean the external surfaces of the instrument regularly to prevent dust accumulation.
2.Clean syringes, flow cells, and other areas prone to contamination following the manufacturer's recommended cleaning method.
Fluidics system maintenance
1.Rinse the flow path after each experiment, especially between mobile phase changes or samples of different properties.
2.Replace ** filters and post-column filters regularly to prevent clogging and cross-contamination.
Pump maintenance
1.Check the condition of the sealing ring of the pump, and replace it in time if it is worn.
2.Perform pump performance checks, such as self-tests or calibrations.
Detector maintenance
1.For UV-Vis detectors, keep the optical window clean and free of stains.
2.Calibrate the sensitivity and baseline stability of the detector as needed.
Temperature control equipment maintenance
1.The column heater should be kept dry and clean to avoid condensation and condensation.
2.Regularly check the accuracy of the temperature control system.
Regular maintenance
1.Schedule regular maintenance and calibration services based on the frequency of use of the instrument and the manufacturer's recommendations.
In summary, each module of the Agilent 1200 Series LC system can be configured independently or combined according to the experimental requirements to build a liquid chromatography system that adapts to the needs of different applications. These precautions and maintenance measures can also be used to extend the life of your Agilent 1200 series LC system and ensure that it continues to deliver accurate and reliable results. In the meantime, please refer to the official manual for detailed operating instructions and maintenance procedures.
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