Laboratory disinfection In plant tissue culture experiments, disinfection of the surface of the space environment is crucial, because contamination of mold spores can have a great impact on the experimental results. This paper will focus on how to effectively disinfect the surface of the space environment of tissue culture laboratories to prevent the contamination of mold spores. First of all, it is important to be clear that contamination of mold spores is one of the most common problems in the tissue culture process. Because mold spores are extremely resistant to stress and can survive in adverse environmental conditions for a long time, measures to prevent their contamination must be comprehensive and thorough.
In tissue culture laboratories, the disinfection of space environment surfaces mainly includes three aspects: air disinfection, surface disinfection and personnel disinfection.
1. Air disinfection.
Air disinfection is an important part of preventing mold spore contamination. Currently, UV disinfection is one of the most commonly used methods. Through ultraviolet irradiation, the DNA structure of mold spores can be destroyed, so as to achieve the purpose of sterilization and disinfection. However, the disadvantage of ultraviolet disinfection is that it has weak penetration, and can only disinfect suspended particles in the air, but cannot completely disinfect aerosols and bacterial corpses in the air. Therefore, in practice, it needs to be used together with other disinfection methods, such as ozone disinfection, chemical gas fumigation, etc.
2. Surface disinfection.
Surface disinfection is mainly to thoroughly clean and disinfect various surfaces in the laboratory, including laboratory countertops, instruments and equipment, doors and windows, floors, etc. In the disinfection process, disinfectants with broad-spectrum sterilization effects should be selected, such as Oak-Taishi disinfectant. At the same time, attention should be paid to the use of appropriate concentrations and action time to avoid ineffective disinfection.
In addition, for some dead corners and crevices that are difficult to clean, some special cleaning tools and methods can be used to deal with them. For example, a vacuum cleaner or pneumatic brush can be used to clean gaps between the benchtop and the floor; For parts such as gaps and handles of doors and windows, you can use alcohol cotton balls to wipe and disinfect.
3. Personnel disinfection.
Personnel disinfection is one of the important measures to prevent mold spore contamination. Experimenters must undergo strict disinfection procedures before entering the laboratory, including changing lab coats, wearing masks and gloves, and pedaling for disinfection pools. In addition, the experimenter should also have regular physical examinations and health examinations to ensure physical health and the hygienic safety of the laboratory environment.
In conclusion, the prevention of mold spore contamination is an important task in plant tissue culture experiments. The risk of contamination of mold spores can be effectively reduced through comprehensive disinfection of air, surfaces, and people. However, there are still some details that need to be paid attention to in practice. For example, it is necessary to maintain the cleanliness and hygiene of the laboratory, and regularly open the windows for ventilation; Thorough cleaning and disinfection of contaminated areas should be carried out in a timely manner; For instruments and equipment that have not been used for a long time, comprehensive cleaning and maintenance should be carried out. Only in this way can we ensure the health and safety of the space environment surface of the tissue culture laboratory and provide guarantee for the smooth progress of plant tissue culture experiments.