Recombinant protein service refers to a biotechnology service that aims to express and purify target proteins on a large scale in vitro through genetic engineering technology to meet the needs of specific proteins in scientific research, drug development and other fields. These proteins usually include recombinant human proteins, animal proteins, and plant proteins. The process of recombinant protein services includes steps such as gene cloning, transfection, expression, purification, and validation. The following is a detailed introduction to the recombinant protein service.
Gene cloning. Gene cloning is the first step in the recombinant protein service, by inserting the gene of the target protein into the appropriate vector to construct a recombinant expression vector. Commonly used vectors include plasmids, viruses, etc., and choosing the right vector can improve the expression efficiency and purity of the target protein.
Transfection. Transfection is the process of introducing a recombinant expression vector into an appropriate host cell, including mammalian cells, insect cells, etc. During the transfection process, factors such as the growth status of the cells and the concentration of the transfection agent need to be considered to ensure the efficient expression of the target protein.
Expression. Expression refers to the process of large-scale synthesis of a target protein in host cells, and optimization of expression conditions can improve the yield and purity of the target protein. Commonly used expression systems include prokaryotic expression systems and eukaryotic expression systems, and the selection of the appropriate expression system can be selected according to the nature and use of the target protein.
Purification. Purification is the process of isolating a protein of interest and removing impurities from an expression system, and commonly used purification methods include affinity chromatography, ion exchange chromatography, gel filtration chromatography, etc. By choosing the purification method reasonably, a high-purity target protein can be obtained.
Verify. Validation refers to the verification of the structure and function of the purified target protein, and commonly used methods include SDS-PAGE, Western blot, mass spectrometry, etc. Validation ensures that the quality of the target protein meets the requirements.
Summary. The recombinant protein service realizes the large-scale expression and purification of target proteins through genetic engineering technology, which provides important support for scientific research and drug development. When selecting recombinant protein services, it is necessary to consider factors such as the nature, use, and quality requirements of the target protein to select the appropriate service provider and service plan.