BRCC3 is a BARD1 complex 3 substrate. It is a protein that interacts effectively with the regulation of DNA repair and transcription factors within the nucleus. The protein encoded by the BRCC3 gene is an acylase with UBA and BRCT domains, which is enriched within the nucleus and forms a complex with DNA damage repair proteins such as BRCA1 and BARD1. BRCC3 plays an important role in DNA repair, mammalian cell cycle control, thymus development, and cellularity. Catalog No. PA1000-8470
As an important bioactive substance, the activity of recombinant BRCC3 protein can be verified by a variety of methods. First, the protein kinase activity of BRCC3 can be determined by traditional enzyme activity analysis, and its activity can be ensured by measuring its substrate protein-specific enzymatic activity against substrates. Secondly, methods such as cellular immunoprecipitation can be used to verify the interaction of BRCC3 with other proteins and observe its effect on biological functions such as DNA damage repair. Finally, bioactive assays, such as DNA damage repair assays, were used to assess the function of brcc3 within cells.
The structure and function of BRCC3 are very complex, with multiple functional domains, including the uba domain and the BRCT domain. The uba domain is often associated with ubiquitin binding and can recognize ubiquitin-modified proteins and promote their deubiquitination. The BRCT domain is a functional domain that interacts with repair proteins and regulates DNA damage repair by interacting with other proteins.
In addition, BRCC3 can also form complexes with other proteins, such as the BARD1-UBA1-BRCC3 complex, which regulates the process of DNA damage repair through a complex network of interactions. Therefore, when studying the biological activity and function of BRCC3, the interaction between BRCC3 and other proteins and signaling pathways need to be considered.
Overall, brCC3 is an important DNA repair protein that plays an important regulatory role within the nucleus. Through the verification of its biological activity and the study of its structure and function, we can gain a deeper understanding of the function of BRCC3 in cells, which provides an important reference and guidance for further research on DNA repair mechanisms and cancer**.