Card9 (Caspase Recruitment Domain-Containing Protein 9) is an important signal transduction protein that belongs to the Caspase Recruitment Domain (Card) family. CARD9 plays a key role in the immune system, regulating the inflammatory response and immune response by participating in signal transduction pathways. The function of card9 is mainly to transmit signals through the binding of its C-terminal card domain to other signaling proteins, thereby regulating inflammatory responses and immune responses.
Catalog No. PA1000-8480
In order to further study the mechanism of action of card9 in inflammation and immunity, researchers often use recombinant protein technology to express and purify card9. Card9 recombinant protein is usually produced on a large scale using expression systems such as Escherichia coli, and then purified by centrifugation, laminar flow column chromatography, etc., and finally a high-purity card9 protein sample is obtained. In this way, a sufficient amount of card9 samples can be obtained for the validation and further study of biological activity.
For the verification of the biological activity of card9, researchers often use a variety of methods. A common approach is to use protein interaction experiments, such as co-immunoprecipitation and GST pull-down assays, to verify the binding of Card9 to other signaling proteins. Another method is to study the regulatory effect of card9 on cell function through cell function experiments, such as cell proliferation and inflammatory factor production. In addition, in vivo experiments can be performed in animal models to observe the effect of card9 on immune response in animals.
The study of the structure and function of card9 is also one of the focuses of scientific researchers. The main structure of Card9 protein includes the card domain at the N-terminus and the shared domain at the C-terminus. The card domain can bind to other signaling proteins, thus forming signaling complexes that transmit signals. The shared domain interacts with other regulatory proteins and participates in the regulation of signal transduction pathways. The study of the structure and function of CARD9 can help us to deeply understand the specific mechanism of action of CARD9 in anti-inflammatory and immunology, and provide an important reference for disease and drug development.
In conclusion, as an important signal transduction protein in the immune system, the verification of its biological activity and structural function are of great significance for understanding the mechanisms of inflammation and immune response. Through the study of card9, we can provide new ideas and methods for disease prevention and prevention. It is hoped that future studies will further reveal the role of card9 in immune regulation.